肝癌患者组织及血清微小RNA-24和微小RNA-629的表达水平及其临床意义  

Expression levels and clinical significance of microRNA-24 and microRNA-629 in tissue and serum of hepatocellular carcinoma patients

作　　者：林洁[1] 黄炳臣[1] 王娟 黄云美[1] 黄小英[1] LIN Jie;HUANG Bing-chen;WANG Juan;HUANG Yun-mei;HUANG Xiao-ying(Clinical Center for Pathological Diagnosis and Research,Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,China)

机构地区：[1]右江民族医学院附属医院临床病理诊断与研究中心,广西百色市533000

出　　处：《广西医学》2020年第14期1775-1778,共4页Guangxi Medical Journal

基　　金：国家自然科学基金(81460423)。

摘　　要：目的探讨肝癌患者组织及血清微小RNA(miR)-24及miR-629的表达水平及其临床意义。方法将67例肝癌患者、31例慢性乙型病毒性肝炎或者乙肝相关性肝硬化患者及46例健康人员,分别设为肝癌组、乙肝组及正常对照组。检测肝癌组癌组织及癌旁正常组织、3组血清标本中miR-24、miR-629的表达水平。分析肝癌组患者血清miR-24、miR-629的表达水平与临床病理指标的相关性。结果肝癌组织中的miR-629及miR-24相对表达水平均高于正常组织(均P<0.05)。正常对照组、乙肝组、肝癌组的血清miR-24、miR-629相对表达水平依次升高(均P<0.05)。肝癌患者miR-629与miR-24的表达水平与血管侵犯情况以及肿瘤分化程度呈正相关(均P<0.05)。结论肝癌患者癌组织及血清中的miR-629、miR-24的表达水平均上调,这可能在肝癌的发生以及慢性肝炎的恶性转化过程中起到重要作用。血清miR-24和miR-629水平的检测或有助于评估肝癌患者的病情。Objective To explore the expression levels and clinical significance of microRNA(miR)-24 and miR-629 in tissue and serum of hepatocellular carcinoma(HCC)patients.Methods Sixty-seven HCC patients,31 patients with chronic hepatitis B or hepatitis B-related cirrhosis and 46 healthy individuals were included as HCC group,hepatitis B group and normal control group,respectively.The expression levels of miR-24 and miR-629 were determined in cancer tissue and para-carcinoma normal tissue of the HCC group,as well as in serum sample of the three groups.The correlation of serum miR-24 and miR-629 expression levels with clinicopathological indexes was analyzed in the HCC group.Results The relative expression levels of miR-629 and miR-24 in HCC tissue were higher than those in the normal tissue(all P<0.05).The relative expression levels of miR-629 and miR-24 increased in the normal control group,the hepatitis B group and the HCC group successively(all P<0.05).In HCC patients,the expression levels of miR-629 and miR-24 positively correlated with the presence of vascular invasion and the degree of tumor differentiation(all P<0.05).Conclusion The expression levels of cancer tissue and serum miR-629 and miR-24 are up-regulated in HCC patients,which may play an important role in the occurrence of HCC and malignant transformation of chronic hepatitis.The detection of serum miR-24 and miR-629 levels may be beneficial to assessment of HCC patients′condition.

关 键 词：肝癌 微小RNA-24 微小RNA-629 乙型病毒性肝炎 恶性转化 

分 类 号：R735.7[医药卫生—肿瘤]