单纯螺钉微创牵引兔下颌骨增加骨量的效果及组织学分析  

Effect and histological analysis of minimally invasive traction of rabbit mandibles with simple screws

作　　者：李泽毓 吴章 马会青 姚玉胜 LI Ze-yu;WU Zhang;MAHui-qing;YAO Yu-sheng(Department of Stomatology,The Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China;Department of Endodontics,The Second Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China)

机构地区：[1]锦州医科大学附属第三医院口腔科,辽宁锦州121000 [2]锦州医科大学附属第二医院牙体牙髓科,辽宁锦州121000

出　　处：《中国临床解剖学杂志》2020年第4期434-437,共4页Chinese Journal of Clinical Anatomy

基　　金：辽宁省科学事业公益研究基金(GY2013-A-015)。

摘　　要：目的探讨应用单纯螺钉旋转牵引兔下颌骨以增加骨量的效果及组织学分析。方法以8只雌性新西兰大白兔为研究对象。随机分左侧或右侧为实验侧,实验侧下颌骨植入2个固定螺钉和1个牵引螺钉,当牵引螺钉向下旋转时,可以给牵引骨块相反的作用力,使截骨断端反向移动,以增加骨量;对照侧不做处理。牵引20 d后,测量实验侧骨增量情况。然后取实验侧牵引区组织及对照侧正常骨组织进行组织学检测,对比两侧组织细胞变化。结果实验侧骨高度增加约2.5 mm;HE染色显示实验侧成骨细胞数量(598.5±130.01)高于对照侧(80.75±14.29);免疫组织化学染色显示实验侧骨形成蛋白BMP-2光密度值(0.139±0.011)高于对照测(0.061±0.013)。结论螺钉旋转可微创实现有效增加骨量。螺钉旋转产生的机械力使受力区组织细胞活性增强,骨形成活跃。Objective To investigate the effect and histological analysis of using simple screws to pull the rabbit mandibles.Methods Eight New Zealand female rabbits were studied and randomly divided into the left side or the right side of the experimental side.The experimental sides of mandibles were implanted with two fixing screws and one traction screw respectively.When the traction screw was rotated downwards,the cutting bone end was moved into the opposite direction to increase the bone mass,while the control side was not treated.After 20 days of traction,the bone mass of the experimental side was measured.Then,the bone tissue of the experimental side and the control side were taken for histological examination.Histiocytic change of the two each sides was compared.Results The bone height of the experimental side increased by nearly 2.5 mm.HE staining showed that the number of osteoblasts on the experimental side(598.5±130.01)were significantly higher than that on the control side(80.75±14.29).Immunohistochemical staining showed that the IOD of the bone morphogenetic protein-2 of the experimental side(0.13±0.011)were significantly higher than that of the control side(0.061±0.013).Conclusions Screws could be minimally and effectively increase bone mass.The mechanical force generated by the rotation of the screws can increase the cells viability of the stressed area,and animate the bone formation.

关 键 词：单纯螺钉 牵引成骨 BMP-2 骨形成 组织学检测 

分 类 号：R782.13[医药卫生—口腔医学]