细胞周期素依赖性蛋白激酶5在垂体腺瘤中的表达及其与血管内皮生长因子关系的研究  

Expression of CDK5 and its relationship with VEGF in pituitary adenoma

作　　者：龚磊 王红云[1] 李丹[1] 张亚卓[1] 谢微嫣 Gong Lei;Wang Hongyun;Li Dan;Zhang Yazhuo;Xie Weiyan(Department of Cell Biology,Beijing Neurosurgical Institute,Capital Medical University,Beijing 100070,China)

机构地区：[1]首都医科大学北京市神经外科研究所细胞生物室,100070

出　　处：《中国微侵袭神经外科杂志》2020年第5期222-226,共5页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：国家自然科学基金(编号:81672495)。

摘　　要：目的研究垂体腺瘤中细胞周期素依赖性蛋白激酶5 (cyclin-dependent kinase 5,CDK5)在垂体腺瘤中的表达,及其与血管内皮生长因子(vascular endothelial growth factor,VEGF)的关系。方法收集8例液氮冻存的垂体腺瘤标本作为垂体腺瘤组,4例正常垂体组织标本作为正常垂体组。应用免疫组织化学染色检测样本中p-CDK5和P35蛋白表达,Western blot检测不同浓度CDK5抑制剂Roscovitine和CDK5 si RNA对GH3细胞系中VEGF表达的影响。结果与正常垂体组比较,垂体腺瘤组VEGF和P35 m RNA表达明显增加(P <0.05),p-CDK5和P35蛋白表达亦增加。随着Roscovitine剂量增加,GH3细胞系中VEGF的表达呈递减趋势;经CDK5 si RNA处理后,VEGF表达降低。结论垂体腺瘤中CDK5的活性明显增加,抑制CDK5活性可降低垂体腺瘤中VEGF的表达。Objective To study the expression of cyclin-dependent kinase 5(CDK5) and its relationship with vascular endothelial growth factor(VEGF) in pituitary adenomas. Methods Eight pituitary adenoma specimens with liquid nitrogen freezing were collected as pituitary adenoma group, and 4 normal pituitary tissues were collected as normal pituitary group. The protein expressions of p-CDK5 and P35 were detected by immunohistochemical staining. The effects of different concentrations of Roscovitine and CDK5 si RNA on the expression of VEGF in GH3 cell lines were detected by Western blotting. Results Compared with normal pituitary group, m RNA expressions of VEGF and P35 were significantly increased(P < 0.05), and the protein expressions of p-CDK5 and P35 were also significantly increased in pituitary adenoma group. With the increase of Roscovitine concentration, the expression of VEGF in GH3 cell line was gradually decreased. After CDK5 si RNA treatment, VEGF expression was also significantly reduced. Conclusions CDK5 activity is significantly increased in pituitary adenoma, and inhibition of CDK5 activity can reduce VEGF expression.

关 键 词：垂体腺瘤 细胞周期素依赖性蛋白激酶5 血管内皮生长因子 免疫组织化学染色 

分 类 号：R739.41[医药卫生—肿瘤]