鱼腥草褐斑病的病原及其生物学特性  

Identification and biological characteristics of pathogen of Houttuynia cordata brown leaf spot

作　　者：高晋 刘思睿 赵致[1,2] 李忠 GAO Jin;LIU Si-Rui;ZHAO Zhi;LI Zhong(Agriculture College of Guizhou University,Guiyang,Guizhou 550025,China;Guizhou Key Laboratory of Medicine Plants Breeding and Cultivation,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]贵州省药用植物繁育与种植重点实验室,贵州贵阳550025

出　　处：《菌物学报》2020年第7期1215-1225,共11页Mycosystema

基　　金：中央引导地方科技发展专项资金项目(黔科中引地[2018]4018);科技部科技惠民计划项目(2013GS520101-08)。

摘　　要：在贵州省施秉县鱼腥草上发生一种新的叶斑类病害,有危害加重趋势。为明确该病的病原菌,本研究采用组织分离法和离体接种法进行了病原菌分离培养和致病性测定,通过形态特征观察及rDNA ITS和Tef1两个核苷酸片段序列分析,将该病菌鉴定为拟盘多毛孢Pestalotiopsis sp.,系鱼腥草上首次报告。对该病原菌的生物学特性进行了研究。发现葡萄糖和蛋白胨最适于该菌菌丝体生长,菌丝体最适生长温度为25℃,最适宜pH 8,病菌在玉米粉培养基上生长最快,光照有利于菌丝体生长。A new leaf spot disease occurred on Houttuynia cordata in Shibing County,Guizhou Province tends to harmful aggravation.Tissue isolation method and in vitro inoculation method were used to isolate and culture the pathogen and determine its pathogenicity.The morphological characteristics and rDNA ITS and Tef1 gene sequence analysis indicated the pathogen was Pestalotiopsis sp.which was first reported on Houttuynia cordata.The biological characteristics of the pathogen were studied.It was found that glucose and peptone were most suitable for the growth of the mycelium.The optimum growth temperature was25°C and the most suitable pH was 8.The pathogen grew well on the corn flour medium.

关 键 词：蕺菜 叶部病害 病原菌 拟盘多毛孢 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]