兔颈动脉粥样硬化斑块光学相干断层扫描参数与胰岛素抵抗的相关性研究  

Study on the relationship between OCT parameters of carotid atherosclerotic plaque and insulin resistance in rabbits

作　　者：原涛 任丽玮 许振坤[1] 张倩 YUAN Tao;REN Li-wei;XU Zheng-kun(The Second Department of Cardiology,Handan First Hospital,Handan 056902,China;不详)

机构地区：[1]邯郸市第一医院心内二科,河北邯郸056902 [2]河北工程大学附属医院耳鼻喉科,河北邯郸056004

出　　处：《中国医学装备》2020年第8期193-197,共5页China Medical Equipment

基　　金：邯郸市科学技术研究与发展计划(1723208066-10)“OCT评价伴有IR的兔颈动脉粥样硬化斑块的研究”。

摘　　要：目的:研究兔颈动脉粥样硬化斑块光学相干断层扫描(OCT)成像参数与胰岛素抵抗的关系。方法:取13只新西兰大白兔,根据喂养法和球囊操作的不同,将其分为模型组(10只)和对照组(3只)。模型组采用高脂饲料喂养+球囊损伤建立兔颈动脉粥样硬化斑块形成模型;对照组采用标准饲料喂养,且无球囊损伤操作。对比两组干预前后甘油三酯(TG)、胆固醇(TC),低密度脂蛋白-胆固醇(LDL-C),高密度脂蛋白-胆固醇(HDL-C)水平;干预后对两组实施OCT检查,对比两组OCT参数;对比两组干预前后空腹血糖(FPG)、空腹胰岛素(FINS)和胰岛素抵抗(HOMA-IR)水平;分析模型组中OCT参数与胰岛素抵抗的关系;对两组大白兔细胞组织行苏木素-伊红(HE)染色,观察病理改变情况。结果:干预后,对照组大白兔血脂、血糖和HOMA-IR均无明显变化;模型组TG、TC、LDL-C、FPG、FINS和HOMA-IR均较干预前升高,而HDL-C较干预前下降,差异有统计学意义(t=6.0171,t=10.4720,t=10.1767,t=10.1154,t=3.6390,t=13.2830,t=7.3978;P<0.05)。干预后模型组TG、TC、LDL-C、FPG、FINS和HOMA-IR均高于对照组,而HDL-C低于对照组,差异有统计学意义(t=3.7915,t=6.2626,t=5.5435,t=5.6212,t=2.3561,t=7.6995,t=7.6985;P<0.05);干预后模型组OCT参数管腔面积小于对照组,新生内膜厚度、新生内膜面积、管腔偏心指数、管腔狭窄程度、纤维帽厚度和脂质池角度均大于对照组,差异有统计学意义(t=8.3386,t=3.8316,t=5.4730,t=5.0383,t=12.4391,t=5.4187,t=6.3807;P<0.05);干预后模型组OCT参数中管腔面积与HOMA-IR呈负相关(r=-0.8523,P<0.05),而OCT参数中新生内膜厚度、新生内膜面积、管腔偏心指数、管腔狭窄程度、纤维帽厚度以及脂质池角度与HOMA-IR均呈正相关(r=0.8448,r=0.8321,r=0.8669,r=0.8947,r=0.8812,r=0.8324;P<0.05);OCT检查与病理检查结果有良好的一致性。结论:高脂饲养+球囊损伤可导致新西兰大白兔糖脂代谢异常,形成颈动脉粥样硬�Objective: To study the relationship between optical coherence tomography(OCT) parameters of carotid atherosclerotic plaque and insulin resistance in rabbits. Methods: Thirteen New Zealand white rabbits were selected and were divided into model group(n=10) and control group(n=3) according to different feeding methods and balloon operation. The model group was fed with high-fat diet and were injured by balloon pulling to establish carotid atherosclerosis plaque formation model, while the control group was fed with standard diet and without being injured by balloon pulling. The triglyceride(TG), total cholesterol(TC), low density lipoprotein-cholesterol(LDL-C), high density lipoprotein-cholesterol(HDL-C) levels before and after intervention between the two groups were compared, respectively. And the fasting blood-glucose(FPG), fasting insulin(FINS) and homeostasis model assessment index of resistance(HOMA-IR) before and after intervention between two groups were compared. And the relationship of OCT parameters and HOMA-IR in model group was analyzed. The Hematoxylin eosin(HE) staining was used in cellular tissue of white rabbits in two groups for observing the pathological changes. Results: The differences of blood lipid, blood glucose and HOMA-IR of control group between pre and post intervention were no significant. And the TG, TC, LDL-C, FPG, FINS and HOMA-IR of model group post intervention were significantly higher than that pre intervention(t=6.0171, t=10.4720, t=10.1767, t=10.1154, t=3.6390, t=13.2830, t=7.3978, P<0.05). The TG, TC, LDL-C, FPG, FINS and HOMA-IR in model group were significantly higher than those in control group after intervention, while HDL-C of model group was significantly lower than that of control group(t=3.7915, t=6.2626, t=5.5435, t=5.6212, t=2.3561, t=7.6995, t=7.6985, P<0.05). The lumen area of OCT parameters of model group was significantly less than that of control group after intervention, while neointima thickness, neointima area, lumen eccentricity index, lumen stenosis degree,

关 键 词：颈动脉粥样硬化 斑块形成 光学相干断层扫描成像(OCT) 胰岛素抵抗 

分 类 号：R-331[医药卫生]