紧密连接蛋白-1介导E-cadherin促进前列腺癌发生进展机制研究  

Study of Mechanism of Claudin-1 Mediated E-cadherin Promotes Prostate Cancer Progression

作　　者：张春霆[1] 卢蓉[2] 张昌文[3] 陈业刚[3] ZHANG Chun-ting;LU Rong;ZHANG Chang-wen(Department of Urology,Zhejiang Jinhua People’s Hospital,Zhejiang(321000),China)

机构地区：[1]浙江省金华市人民医院泌尿外科,金华321000 [2]浙江省金华市人民医院妇产科,金华321000 [3]天津医科大学第二医院泌尿外科,天津300211

出　　处：《中国中西医结合外科杂志》2020年第4期654-657,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：浙江省金华市科技局重点计划资助项目(2017-3-016);浙江省医药卫生科技计划项目(2019-KY766)。

摘　　要：目的:探讨紧密连接蛋白(Claudin)-1和E-钙黏蛋白(E-cadherin)在前列腺癌组织中的表达及其在前列腺癌中的作用机制。方法:应用免疫组织化学SP法检测72例前列腺癌组织和42例前列腺增生组织中Claudin-1和E-cadherin的表达状况,研究二者的表达状况与前列腺癌临床病理特征之间的关系,分析二者在前列腺癌中表达的相关性。结果:Claudin-1在前列腺癌组织中表达阳性率为63.89%(46/72),显著高于前列腺增生组织的33.33%(14/42),E-cadherin在前列腺癌中表达率为13.89%(10/72),低于在前列腺增生中E-cadherin的表达率90.48%(38/42),差异有统计学意义(均P<0.01)。Claudin-1和E-cadherin蛋白表达与前列腺癌Gleason评分、临床分期有关(P<0.05),与PSA增高无关(P>0.05)。Spearman相关分析显示Claudin-1和E-cadherin表达呈负相关(r=–0.68)。结论:Claudin-1和E-cadherin在前列腺癌中异常表达,它们的异常表达可能共同参与了前列腺癌的发生、进展,联合检测它们有望提高对前列腺癌预后的评估。Objective To investigate the expressions and relation of Claudin-1 to E-cadherin proteins and their mechanisms in prostate cancer.Methods Immunohistochemical SP method was used to detect the expression of Claudin-1 and E-cadherin in 72 cases with prostate cancer and 42 cases with prostate hyperplasia tissue.The relationship was studied between the clinical pathological characteristics of prostate cancer and its expression.Results The positive rate of Claudin-1 expression in prostate cancer tissues was 63.89%(46/72),which is significantly higher than that of 33.33%(14/42)in prostate hyperplasia tissues.The positive rate of E-cadherin expression was 13.89%(10/72)in prostate cancer and 90.48%(38/42)in prostate hyperplasia,which the difference was statistically significant(P<0.01).The positive rate of Claudin-1 and E-cadherin protein expression were associated with Gleason grade,clinical stage,but they were not related to PSA in prostate cancer(P<0.05).Abnormal expression of Claudin-1 and E-cadherin showed a negative correlation with Spearman correlation analysis in prostate cancer,with a correlation coefficient of r=-0.68.Conclusion Abnormal expression of Claudin-1 and E-cadherin in prostate cancer may be involved in the occurrence and progression of prostate cancer.Combined detection of Claudin-1 and E-cadherin may improve the prognosis assessment of prostate cancer.

关 键 词：前列腺肿瘤 紧密连接蛋白-1 E-钙粘素 免疫组织化学 

分 类 号：R737.25[医药卫生—肿瘤]