玉米须多糖对高糖诱导的肾小球系膜细胞增殖及炎症因子表达的影响  

Effects of Stigma maydis Polysaccharide on High Glucose-induced Proliferation of Mesangium Cells and Expression of Inflammatory Factors

作　　者：曲萌[1] 侯勤龙 翁诗雅 高润泽 宋宇[1] 董志恒[1] QU Meng;HOU Qinlong;WENG Shiya;GAO Runze;SONG Yu;DONG Zhiheng(Medical College of Beihua University,Jilin 132013,China)

机构地区：[1]北华大学医学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2020年第4期461-465,共5页Journal of Beihua University（Natural Science）

基　　金：吉林省教育厅科学技术研究项目(201666);吉林省中医药管理局科技项目(2018109);北华大学科研平台项目(20140301);国家级大学生创新创业训练计划项目(201810201021);北华大学研究生创新计划项目(2019070).

摘　　要：目的探讨玉米须多糖(Stigma Maydis polysaccharide,SMP)对高糖刺激下大鼠HBZY-1细胞增殖、炎症因子MCP-1、IL-6在基因及蛋白水平表达的影响.方法将实验细胞分为正常对照组(NG)、高糖组(HG)、高糖加不同浓度SMP组(SMP35组、SMP70组)4组,以35、70μg/mL两个质量浓度的SMP干预高糖刺激下的大鼠HBZY-1细胞,应用四甲基偶氮唑盐(MTT)光吸收法观察细胞增殖情况;应用RT-qPCR法检测HBZY-1细胞中MCP-1、IL-6 mRNA表达;应用Western Blot法检测HBZY-1细胞中MCP-1、IL-6蛋白表达.结果与NG组比较,HG组HBZY-1增殖加快(P<0.01),MCP-1、IL-6两指标的mRNA和蛋白表达均显著增加(P<0.01);与HG组比较,各质量浓度的SMP均可抑制高糖对大鼠HBZY-1细胞的增殖(P<0.01),mRNA和蛋白水平可下调MCP-1、IL-6的表达(P<0.01),且呈剂量依赖效应.结论SMP可能通过下调炎症因子MCP-1、IL-6表达,抑制高糖诱导的大鼠HBZY-1增殖,从而达到延缓糖尿病肾病发生、发展的作用.Objective To investigate the effects of Stigma maydis polysaccharide(SMP)on the proliferation of HBZY-1 cells stimulated by high glucose and the expression of monocyte chemoattractant protein-1(MCP-1)and interleukin-6(IL-6)at gene and protein levels.Method The experimental cells were divided into normal control group(NG),high glucose group(HG)and high glucose group with different concentration of SMP(SMP35 group and SMP70 group).The rats HBZY-1 cells were treated with SMP at 35 and 70μg/mL concentrations,the proliferation of rat HBZY-1 cells under high glucose stimulation was observed by the light absorption method of tetramethylazolium salt(MTT).The mRNA expression of MCP-1 and IL-6 in HBZY-1 cells was detected by RT-qPCR.The protein expression of MCP-1 and IL-6 in HBZY-1 cells was detected by Western Blot.Results Compared with the normal control group(NG group),HBZY-1 proliferation was accelerated in the high glucose group(HG group)(P<0.01),and mRNA and protein expressions of MCP-1 and IL-6 were significantly increased(P<0.01).Compared with HG group,the promotion effect of high glucose on the proliferation of rat HBZY-1 cells was inhibited in all SMP groups(P<0.01),and the expressions of MCP-1 and IL-6 were down-regulated at mRNA and protein levels(P<0.01),showing a dose-dependent effect.Conclusion SMP may play a role in delaying the occurrence and development of diabetic nephropathy by down-regulating the expression of inflammatory factors MCP-1 and IL-6 and inhibiting the proliferation of high-glucose-induced HBZY-1 cells in rats.

关 键 词：玉米须多糖 糖尿病肾病 肾小球系膜细胞 细胞增殖 炎症因子 

分 类 号：R285.5[医药卫生—中药学]