结肠癌相关转录因子1在非小细胞肺癌组织的表达及其对细胞增殖和迁移的影响  

Expression of colon cancer associated transcript 1 in non-small cell lung cancer and its effect on proliferation and migration

作　　者：刘青锋 魏立 张志飞[2] 候广杰 汤金星 赵璞 张全 杨军峰 何苡 Liu Qingfeng;Wei Li;Zhang Zhifei;Hou Guangjie;Tang Jinxing;Zhao Pu;Zhang Quan;Yang Junfeng;He Yi(Department of Thoracic Surgery,Henan Provincial People’s Hospital,People’s Hospital of Zhengzhou University,School of Clinical Medicine,Henan University,Zhengzhou 450003,China;International Medical Center,Henan Provincial People’s Hospital,Zhengzhou 450003,China)

机构地区：[1]河南省人民医院郑州大学人民医院河南大学人民医院胸外科,450003 [2]河南省人民医院国际医疗中心,郑州450003

出　　处：《中华实验外科杂志》2020年第6期1141-1144,共4页Chinese Journal of Experimental Surgery

基　　金：2019年河南省医学科技攻关计划省部共建项目(SB201901079)。

摘　　要：目的观察结肠癌相关转录因子1(CCAT1)在非小细胞肺癌中的表达及其对细胞增殖和迁移的影响。方法收集2017年8月至2019年8月河南省人民医院手术摘除的102例非小细胞肺癌组织和对应的癌旁组织作为研究对象。采用荧光定量聚合酶链反应(PCR)分析非小细胞肺癌组织和癌旁组织中CCAT1表达水平。采用RNA干扰技术在A549细胞(购自中国科学院上海细胞库)建立CCAT1敲降稳定细胞株和对照细胞株,命名为CCAT1 KD组和对照组。采用细胞计数试剂盒(CCK-8)和5-乙炔基-2’脱氧尿嘧啶核苷(EdU)测定两组细胞增殖;采用Transwell分析两组细胞迁移;采用蛋白质印迹法(Western blot)分析细胞增殖和迁移相关蛋白的表达水平。组间比较采用t检验。结果与癌旁组织CCAT1水平(1.26±0.28)比较,非小细胞肺癌组织中CCAT1水平(3.47±0.31)显著增加,差异有统计学意义(t=3.091,P<0.05)。与对照组A549细胞CCAT1表达水平(1.09±0.15)比较,CCAT1 KD组细胞CCAT1表达水平(0.37±0.09)显著下调,差异有统计学意义(t=4.100,P<0.05)。与对照组细胞吸光度(A)值(1.84±0.26)比较,CCAT1 KD组细胞A值(0.98±0.14)显著下降,差异有统计学意义(t=3.719,P<0.05)。与对照组细胞EdU阳性率[(70.26±8.92)%]比较,CCAT1 KD组细胞EdU阳性率[(30.41±5.89)%]显著下降,差异有统计学意义(t=3.201,P<0.05)。与对照组细胞迁移数量[(128.44±9.17)个]比较,CCAT1 KD组细胞迁移数量[(59.19±8.01)个]显著下降,差异有统计学意义(t=3.172,P<0.05)。与对照组细胞细胞核增殖抗原(Ki-67)、增殖细胞核抗原(PCNA)和黏着斑激酶(FAK)表达水平(1.18±0.16、1.21±0.20、1.08±0.19)比较,CCAT1 KD组细胞Ki-67、PCNA和FAK表达水平(0.25±0.14、0.38±0.09、0.31±0.13)显著下降,差异有统计学意义(t=3.207、3.801、3.510,P<0.05)。结论CCAT1在非小细胞肺癌组织中呈高表达,并参与非小细胞肺癌的增殖和迁移。Objective To observe the expression of colon cancer associated transcript 1(CCAT1)in non-small cell lung cancer(NSCLC)and its effect on cell proliferation and migration.Methods Totally,102 cases of NSCLC and corresponding adjacent tissues were collected from August 2017 to August 2019 as research objects.The expression of CCAT1 in NSCLC and adjacent tissues was analyzed by fluorescence quantitative polymerase chain reaction(PCR).CCAT1 knockdown stable cell line and control cell line in A549 cells were established by RNA interference technology,named CCAT1 KD group and control group respectively.The cell proliferation was analyzed by cell counting kit-8(CCK-8)and 5-Ethynyl-2’-deoxyuridine(EdU).The cell migration was analyzed by Transwell.The expression levels of cell proliferation and migration related proteins were analyzed by Western blotting.Results Compared with the cancerous adjacent tissues(1.26±0.28),the level of CCAT1(3.47±0.31)in NSCLC tissues significantly increased(t=3.091,P<0.05).Compared with the control group,the expression level of CCAT1 in A549 cells(1.09±0.15)significantly decreased(0.37±0.09,t=4.100,P<0.05).Compared with the control group(1.84±0.26),the cell proliferation of CCAT1 KD group(0.98±0.14)significantly decreased(t=3.719,P<0.05).Compared with the control group[(70.26±8.92)%],the positive rate of EdU in CCAT1 KD group[(30.41±5.89)%]significantly decreased(t=3.201,P<0.05).Compared with the control group(128.44±9.17),the number of migrating cells in CCAT1 KD group(59.19±8.01)significantly decreased(t=3.172,P<0.05).Compared with the control group(1.08±0.16,1.21±0.20,1.18±0.19),the expression levels of proliferation cell nuclear antigen(Ki-67),PCNA and focal adhesion kinase(FAK)in CCAT1 KD group(0.38±0.14,0.25±0.09,0.31±0.13)significantly decreased(t=3.207,3.801,3.510,P<0.05).Conclusion CCAT1 is highly expressed in NSCLC tissues and participates in the proliferation and migration of NSCLC.

关 键 词：结肠癌相关转录因子1 非小细胞肺癌 增殖 迁移 

分 类 号：R734.2[医药卫生—肿瘤]