钙激活中性蛋白酶抑制剂对肝癌细胞生物学行为及Src基因表达的影响及意义  

Effects of significance of calcium-activated neutral protease inhibitor on biological behavior and expression of Src gene in hepatocellular carcinoma cells

作　　者：张帅民[1] 张毅[1] 刘振华[1] 汤可立[1] 陈根 Zhang Shuaimin;Zhang Yi;Liu Zhenhua;Tang Keli;Chen Gen(Department of Organ Transplantation,Guizhou People's Hospital,Guiyang,Guizhou,550002,China)

机构地区：[1]贵州省人民医院器官移植科,贵州贵阳550002

出　　处：《当代医学》2020年第24期98-100,共3页Contemporary Medicine

摘　　要：目的观察钙激活中性蛋白酶-2(Calpain-2)抑制剂ALLM对不同迁移能力肝癌细胞系侵袭迁移能力及Src基因表达的影响,并探索ALLM抑制肝癌的机制。方法常规细胞培养肝癌细胞系MHCC97-H、MHCC97-L、HepG2及正常人肝细胞HL-7702,经高浓度抑制剂ALLM处理后采用划痕实验、Transwell实验及western-bolt方法观察3株肝癌细胞及人正常肝细胞侵袭迁移能力及肝癌细胞中Src基因表达变化。结果与对照组比较,经ALLM处理的MHCC97-H、MHCC97-L的迁移距离、穿膜细胞的吸光度OD值差异具有统计学意义(P<0.05);转移性MHCC97-H、MHCC97-L肝癌细胞系Src蛋白的表达量显著下降(P<0.01);HepG2及正常人肝细胞HL-7702与转移性肝癌细胞迁移距离、穿膜细胞吸光度OD值及Src表达量比较差异具有统计学意义(P<0.05)。结论ALLM抑制Calpain-2的活性使肝癌细胞的迁移和侵袭能力显著下降,其机制可能与抑制原癌基因Src的表达和Src蛋白的活性有关。Objective To investigate the effects of Calpain-2 inhibitor ALLM on invasion,migration ability and expression of Src gene in hepatocellular carcinoma cell lines with different migration abilities.Methods Conventional cell culture MHCC97-H,MHCC97-L,HepG2 and normal human liver cells HL-7702 cells.Observed the changes of three hepatocellular carcinoma cells and normal liver cells in the invasion,migration and expression of Src gene by the method of wound healing assay,transwell and Western-bolt,after treated by ALLM of high concentration.Results Compared with the control group,the migration distance of the ALLM-treated MHCC97-H,MHCC97-L,and the absorbance OD of the transmembrane cells were significantly difference(P<0.05).The expression of metastatic MHCC97-H,MHCC97-L liver cancer cell lines of Src protein weresignificantly decreased(P<0.01).Compared with HepG2 and normal human hepatocyte HL-7702,the migration distance,absorbance OD value of transmembrane cells,and Src expression was significantly difference in metastatic liver cancer cells(P<0.05).Conclusion ALLM inhibits the activity of Calpain-2 and significantly reduces the migration and invasion ability of liver cancer cells.The mechanism may be related to the inhibition of the expression of proto-oncogene Src and the activity of Src protein.

关 键 词：肝癌细胞 侵袭 转移 Src原癌基因 

分 类 号：R735.7[医药卫生—肿瘤]