基于G-四链体-血红素和链式放大反应的基因检测传感器的构建  

Construction of a Sensor for Gene Detection Based on G-quadruplex-Hemin and Chain Amplification Reaction

作　　者：孙延修[1] 孙笑凡 SUN Yan-xiu;SUN Xiao-fan(Department of Basic Courses,Shenyang Institute of Technology,Fushun 113122,China;Wuxi Yaoming Biotechnology Co.,Ltd,Wuxi 214122,China)

机构地区：[1]沈阳工学院基础课部,辽宁抚顺113122 [2]无锡药明生物技术股份有限公司,江苏无锡214122

出　　处：《分析测试学报》2020年第6期774-778,共5页Journal of Instrumental Analysis

基　　金：中国烟草总公司标准项目(2018QBZ08)。

摘　　要：该文以特殊设计的DNA序列为捕获探针,以G-四链体-血红素复合物作为信号分子,利用链式反应实现目标DNA的灵敏检测。在目标DNA存在时,捕获探针与目标DNA相互识别,同时目标DNA能与辅助探针发生连续的链式反应,从而在电极表面引入大量G-四链体结构。血红素存在下,G-四链体可与血红素结合形成具有很强电化学信号的G-四链体-血红素复合物。用差分脉冲伏安法(DPV)扫描得到的电化学信号与体系中的目标DNA浓度存在对应关系,从而实现对目标DNA的检测。在各组分浓度最适的情况下,电流响应值与目标DNA浓度在0.01~10 pmol/L内具有良好的线性关系,检出限可达8 fmol/L。该传感器灵敏度高、特异性好,具有良好的应用前景。In this study,a DNA biosensor was constructed for the sensitive detection of target DNA by chain reaction,with a specially-designed DNA sequence as capture probe and a G-quadruplex-hemin complex as signal molecule.In the presence of target DNA,the capture DNA and the target DNA could recognize each other,while the target DNA could have a continuous chain reaction with the assistant probe,leading to the formation of a large number of G-quadruplex on the electrode surface.In the presence of hemin,the G-quadruplex combines with the hemin to form a G-quadruplex-hemin complex,which emits a strong electrochemical signal.Therefore, the detection of target DNA could be realized as the electrochemical signal obtained by differential pulse voltammetry(DPV) has a corresponding relationship with concentration of the target DNA in the system.Under the optimal conditions for the concentration of each component,the DPV current response has a good linear relationship with the target DNA concentration in the range of 0.01-10 pmol/L,and the minimum detection limit reaches to 8 fmol/L.With the advantages of high sensitivity and good specificity,the sensor has a good application prospect.

关 键 词：DNA生物传感器 G-四链体 血红素 基因检测 

分 类 号：O657.1[理学—分析化学] Q343.11[理学—化学]