脱落酸对盐胁迫下香椿幼苗离子吸收和光合作用的影响  

Effects of Abscisic Acid on Ion Absorption and Photosynthesis of Toona sinensis Seedlings under Salt Stress

作　　者：姚侠妹 偶春 张源丽 杨丽梅 徐梦 王群群 屈长青[2] Yao Xiamei;Ou Chun;Zhang Yuanli;Yang Limei;Xu Meng;Wang Qunqun;Qu Changqing(Anhui Jianzhu University,Hefei 230022,P.R.China;Engineering Technology Research Center of Anti-aging Chinese Herbal Medicine,Fuyang Normal University)

机构地区：[1]安徽建筑大学,合肥230022 [2]抗衰老中草药安徽省工程技术研究中心(阜阳师范大学)

出　　处：《东北林业大学学报》2020年第8期27-32,共6页Journal of Northeast Forestry University

基　　金：安徽省高校自然科学研究重点项目(KJ2018A0349);阜阳市政府-阜阳师范学院横向合作一般项目(XDHX201713);安徽省哲学社会科学规划项目(AHSKQ2019D097);安徽省高校质量工程项目(2019jyxm0268,2019sjjd28);安徽省新农科研究与改革实践项目(皖教秘高〔2020〕60号)。

摘　　要：以香椿幼苗为试验材料,采用水培方式,研究不同浓度脱落酸(0、1、10和100μmol/L ABA)对盐(150 mmol/L NaCl)胁迫下香椿幼苗生长、离子质量分数和光合作用的影响。结果显示:与CK相比,盐胁迫明显抑制了香椿幼苗的生长,增加叶片和根系中Na+质量分数,降低K+、Ca ^2+和Mg^ 2+的积累,叶片净光合速率(P n)降低。施加适宜浓度的ABA处理后,香椿幼苗生长受抑情况得到缓解,Na+质量分数降低,K+、Ca^ 2+和Mg^ 2+质量分数增加,w(K+)∶w(Na+)、w(Mg^ 2+)∶w(Na+)和w(Ca^ 2+)∶w(Na+)比值提高,叶片净光合速率(P n)、蒸腾速率(T r)、气孔导度(G s)、胞间CO 2摩尔分数(C i)和水分利用率(W UE)上升,气孔限制值(L s)降低,同时叶绿素质量分数提高,丙二醛(MDA)质量摩尔浓度降低。研究结果表明,NaCl胁迫下添加外源适宜浓度的ABA,能够有效地抑制盐胁迫下香椿叶片和根系对Na+的吸收,改善香椿幼苗中的离子平衡,促进营养吸收,同时增强植株光合作用,降低膜质过氧化水平,从而增强香椿幼苗对盐胁迫的适应性。其中,香椿幼苗在浓度为10μmol/L ABA处理时的缓解效果最佳。To assess the role of exogenous abscisic acid (ABA) in Toona sinensis under salt stress, T. sinensis seedlings exposed to 150 μmol/L NaCl were treated with different concentrations (0, 1, 10 and 100 μmol/L)of ABA, and the growth, ion content and photosynthesis of seedlings under 150 mmol/L NaCl were studied. The results showed that: salt stress significantly inhibited the growth of T. sinensis seedlings with the increased Na + content, the decreased accumulation of K +, Ca 2+ and Mg 2+ in leaves and roots and the decreased net photosynthetic rate ( P n) of leaves compared with the control. Under salt stress, exogenous appropriate concentration of ABA alleviated the growth inhibition of the seedlings, remarkably reduced the Na + content in roots and leaves, increased the contents of K +, Ca 2+ and Mg 2+ , and enhanced values of K +/Na +, Mg 2+ /Na + and Ca 2+ /Na +. The net photosynthetic rate ( P n), transpiration rate ( T r), stomatal conductance ( G s), intercellular CO 2 concentration ( C i), water use efficiency ( W UE ) and chlorophyll contents of leaves were increased with the decreased stomatal limit value ( L s), while MDA content were decreased. Adding exogenous suitable concentration of ABA under NaCl stress could effectively inhibit the absorption of Na + in leaves and roots of T. sinensis under salt stress, improve the ion balance in seedlings, promote nutrient absorption, enhance plant photosynthesis and reduce membrane peroxidation level, which enhanced the adaptability of T. sinensis seedlings to salt stress. Among them, T. sinensis seedlings treated with 101μmol/L abscisic acid had the best relief-effect.

关 键 词：香椿 脱落酸 盐胁迫 离子吸收 光合作用 

分 类 号：S644.4[农业科学—蔬菜学]