白蚁肠道木质素降解菌分离鉴定及其降解特性  

Isolation and Identification of Lignin-degrading Bacteria from the Gut of Termite and Their Degradation Characteristics

作　　者：李锋 黄庶识[2] LI Feng;HUANG Shu-shi(School of Chemistry and Chemical Engineering,Qiannan Normal College for Nationalities,Duyun 558000;Guangxi Academy of Sciences,Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynethesis Chemistry,Nanning 530007)

机构地区：[1]黔南民族师范学院化学化工学院,都匀558000 [2]广西科学院广西海洋天然产物与组合生物合成重点实验室,南宁530007

出　　处：《生物技术通报》2020年第8期61-68,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31560017);广西自然科学基金重点项目(2014GXNSFDA118012);黔南民族师范学院引进高层次人才研究专项(qnsy2019)。

摘　　要：以白蚁肠道为菌株来源,富集分离木质素降解细菌,并进一步解析木质素降解过程。从白蚁肠道中筛选木质素降解菌,对其进行16S rRNA基因序列鉴定,通过FTIR和TGA等手段解析了该菌对木质素的降解特性。结果显示,菌株MP-132经鉴定为解鸟氨酸拉乌尔菌,以碱木质素为唯一碳源时,培养7 d后,木质素降解率可达53.2%,漆酶和木质过氧化物酶活力最大分别为32 U/L和105.6 U/L。该菌能够使苯胺蓝和天青B脱色。此外,FTIR和TGA分析结果表明细菌生物处理改变了木质素结构。较为系统揭示了菌株MP-132的木质素降解特性,为木质素资源化利用方面提供优良菌株资源。The objectives of this work are to enrich and isolate lignin-degrading bacteria from the gut of termite,and to study their degradation characteristics.A lignin-degrading bacterium was first isolated from the gut of a termite species,Microtermes pakistanicus,and then identified by 16S rRNA gene sequence analysis for their phylogeny property.Further,with a variety of evaluation methods,such as FTIR and TGA,the degradation characteristics of lignin for the strain were analyzed.As results,a strain MP-132 was identified as Raoultella ornithinolytica.The degradation efficiency was up to 53.2%after 7 d culture when alkaline lignin was used as sole carbon source.Laccase and lignin peroxidase peroxidase reached maximum activities 32 U/L and 105.6 U/L,respectively.Moreover,strain MP-13 led the azure B and aniline blue decolorized.The results from the FTIR and TGA analysis showed that strain MP-132 uniquely presented an attractive capability to deconstruct lignin components.In conclusion,this work systematically reveals the degradation characteristics of strain MP-132,which may provide fine germplasm resources for the biodegradation of lignin.

关 键 词：木质素 拉乌尔菌 白蚁 降解 

分 类 号：X172[环境科学与工程—环境科学]