利用纤维床生物反应器发酵生产链霉素  

Production of Streptomycin by Fermentation in Fibrous-Bed Bioreactor

作　　者：贾啸静 杨尚天[2] JIA Xiaojing;YANG Shangtian(Huasheng Company of NCPC,Hebei Industrial Enterprise Experimental Research Center,Shijiazhuang 052160,China;Department of Chemical and Biomolecular Engineering,Ohio State University,Ohio 43221,USA)

机构地区：[1]华北制药华胜有限公司河北省工业企业试验研究中心,河北石家庄052160 [2]美国俄亥俄州立大学化学与生物分子工程系,俄亥俄43221

出　　处：《化学与生物工程》2020年第8期41-44,67,共5页Chemistry & Bioengineering

基　　金：河北省人社厅提供在俄亥俄州立大学访学研究期间的资金支持;华北制药华胜有限公司提供在俄亥俄州立大学访学研究期间的资金支持

摘　　要：利用纤维床生物反应器(FBB)发酵生产链霉素,对发酵培养基氮源进行了优化,并与传统搅拌发酵进行了比较。结果表明,以可溶性有机氮源大豆蛋白胨作为发酵培养基氮源,并添加一定量蔗糖,可以完全替代黄豆粉,在1 LFBB中发酵96 h时链霉素效价达到5567 U·mL^-1。在整个发酵过程中,所有菌体都被固定在纤维床反应器上,而发酵液保持澄清,这不仅有利于从无菌体的发酵液中进行产物的提取和纯化,也可以重复利用菌体生产次级代谢产物,从而缩短发酵周期,进一步提高链霉素在发酵过程中的体积生产率。We investigated the production of Streptomycin by fermentation in fibrous-bed bioreactor(FBB),optimized the nitrogen source of fermentation medium,and compared with traditional agitating fermentation.The results show that soybean powder can completely be replaced by adopting soy peptone,a soluble organic nitrogen source,as the nitrogen source of the fermentation medium and simultaneously adding a certain amount of sucrose.The titer of Streptomycin reaches 5567 U·mL^-1 after fermentation for 96 h in 1 L FBB.In the whole fermentation process,all the bacteria are immobized on the fibrous-bed reactor,and the fermentation broth remains clear,which not only is conducive to the extraction and purification of products from the fermentation broth without bacteria,but also can reuse the bacteria to produce secondary metabolites,so as to shorten the fermentation cycle and further improve the volumetric productivity of Streptomycin in the fermentation process.

关 键 词：灰色链霉菌 链霉素 纤维床生物反应器 发酵 

分 类 号：TQ465[化学工程—制药化工] R978.1[医药卫生—药品]