超高效液相色谱-串联质谱法同时测定红曲类保健食品中美伐他汀和去羟基洛伐他汀  

Simultaneous Determination of Mevastatin and Dehydro Lovastatin in Monascus Kinds of Health Food by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry

作　　者：严俊 颜琳琦 胡磊 陈岑 徐柏杨 周明昊 YAN Jun;YAN Lin-qi;HU Lei(Zhejiang Institute for Food and Drug Control,Hangzhou,Zhejiang 310000)

机构地区：[1]浙江省食品药品检验研究院,浙江杭州310000

出　　处：《安徽农业科学》2020年第16期192-194,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立了同时检测红曲类保健食品中美伐他汀、去羟基洛伐他汀的超高效液相色谱-串联质谱法。[方法]样品经甲醇超声提取,采用Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm),流动相为乙腈-0.1%甲酸/水溶液(75∶25),流速0.2 mL/min,以电喷雾离子源正离子多反应模式(MRM)进行检测。[结果]2种目标物在4 min内得到良好分离。美伐他汀和去羟基洛伐他汀分别在6.1~606.6和4.8~483.5 ng/mL范围内与峰面积呈良好的线性关系(r=1),低、中、高3个添加水平的回收率在97%~105%,RSD<3.0%,检出限分别为17、15μg/kg。[结论]该方法简单、快速、灵敏、准确,可用于红曲类保健食品中美伐他汀和去羟基洛伐他汀的同时检测。[Objective] To establish a method for simultaneous determination of mevastatin and dehydro lovastatin in monascus kinds of health food based on ultra-high performance liquid chromatography-tandem mass spectrometry. [Method] The samples were extracted by methanol. With acetonitrile and 0.1 % formic acid(75∶25) as mobile phase at a flow rate of 0.2 mL/min, the determination was conducted on Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm, 1.7 μm) column by tandem mass spectrometry in positive ESI mode under multiple reaction monitoring(MRM) mode. [Result] The two targets were well separated within 4 min. The calibration curve of mevastatin and dehydro lovastatin was linear in the concentration range of 6.1-606.6 and 4.8-483.5 ng/mL(r=1), respectively. The average recovery were in range of 97%-105% at low, modern and high added levels, while RSD was less than 3.0%. The limit of detection were 17, 15 μg/kg, respectively. [Conclusion] The method is convenient, rapid, sensitive and accurate, which is suitable for simultaneous detection of mevastatin and dehydro lovastatin in monascus kinds of health food.

关 键 词：超高效液相色谱-串联质谱法 美伐他汀 去羟基洛伐他汀 红曲类保健食品 同时测定 

分 类 号：TS207.3[轻工技术与工程—食品科学]