川陈皮素抑制胃癌SGC-7901细胞侵袭能力的机制探讨  

Study on the mechanism of nobiletin inhibiting the invasiveness of SGC-7901 gastric cancer cells

作　　者：杨雪竹 张浩[2] 崔西玉 苏沛珠 李兆滔 Yang Xuezhu;Zhang Hao;Cui Xiyu;Su Peizhu;Li Zhaotao(Gastroenterology Department,Foshan First People's Hospital(Foshan Hospital Affiliated to Sun Yat-sen University),Guangdong Foshan 528000,China;Oncology Department,People's Hospital of Wuhan University,Hubei Wuhan 430061,China)

机构地区：[1]佛山市第一人民医院(中山大学附属佛山医院)消化内科,广东佛山528000 [2]武汉大学人民医院肿瘤科,湖北武汉430061

出　　处：《现代肿瘤医学》2020年第18期3099-3104,共6页Journal of Modern Oncology

基　　金：广东省医学技术科学研究基金项目(编号:A2018291)。

摘　　要：目的:观察川陈皮素(nobiletin,Nob)对胃癌SGC-7901细胞侵袭能力的影响,并探讨其可能的作用机制。方法:不同浓度的川陈皮素(0、30、60、120 mg/L)体外处理SGC-7901细胞。CCK8法检测细胞增殖能力;Transwell小室法检测细胞侵袭能力的改变;免疫印迹法检测细胞上皮间质转化(epithelial-mesenchymal transition,EMT)标志物(E-cadherin、Vimentin、Fibronectin)蛋白、MMP-9、STAT3、p-STAT3蛋白表达的改变。结果:CCK8和Transwell小室法检测结果显示,与对照组(0 mg/L)比较,川陈皮素可显著抑制SGC-7901细胞的增殖和侵袭能力;免疫印迹结果显示,随着川陈皮素浓度的增加,SGC-7901细胞E-cadherin蛋白表达逐渐上调,同时Vimentin、Fibronectin蛋白的表达量降低,MMP-9蛋白表达下调,STAT3总量未发生变化,但p-STAT3表达逐渐降低。结论:川陈皮素可降低胃癌SGC-7901细胞侵袭能力,其机制可能为通过抑制STAT3通路,继而抑制EMT。Objective:To investigate the effect of nobiletin(Nob)on the cell invasiveness in SGC-7901 gastric cancer cells and explore the possible mechanisms.Methods:SGC-7901 cells were cultured in vitro.After treatment by nobiletin at different concentrations(0,30,60 and 120 mg/L)respectively,cell proliferation was detected by CCK8 assay and the invasion of SGC-7901 cells was tested by Transwell assay.Western blot was used for E-cadherin,Vimentin,Fibronectin,MMP-9,STAT3 and p-STAT3 protein analysis.Results:Results of CCK8 showed that cell proliferation was inhibited by nobiletin significantly and Transwell experiments revealed that the number of SGC-7901 cells treated by nobiletin passing through the basement membrane was less than that of the control group(P<0.05),suggesting that nobiletin could inhibit the capacity of cell invasion.Nobiletin could promoted the expressions of E-cadherin,and suppressed the expressions of Vimentin,Fibronectin,MMP-9 and p-STAT3 in a dose-dependent manner.Conclusion:Nobiletin could inhibit the capacity of cell invasion,and the effects of antitumor may be associated with inhibition of the STAT3 signaling pathway,thus inhibiting of epithelial-to-mesenchymal transition in gastric cancer cell line SGC-7901 cells.

关 键 词：川陈皮素 SGC-7901细胞 侵袭 上皮间质转化 STAT3信号通路 

分 类 号：R735.2[医药卫生—肿瘤]