2018年德阳市胃肠炎暴发中诺如病毒分子病原学特征研究  

Molecular etiological characteristics of the gastroenteritis outbreak associated with Norovirus infection in Deyang city,2018

作　　者：曹冉冉 何杨[3] 吴晓宇[3] 马小珍 任敏[2] Cao Ranran;He Yang;Wu Xiaoyu;Ma Xiaozhen;Ren Min(Sichuan University,Chengdu 610041,China;Sichuan Center for Disease Control and Prevention,Chengdu 610041,China;Deyang Municipal Center for Disease Control and Prevention,Deyang 618000,China)

机构地区：[1]四川大学,成都610041 [2]四川省疾病预防控制中心,成都610041 [3]德阳市疾病预防控制中心,618000

出　　处：《中华实验和临床病毒学杂志》2020年第3期284-288,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科技重大专项(2017ZX10103010-006)。

摘　　要：目的了解2018年德阳市急性胃肠炎(acute gastroenteritis,AGE)暴发疫情中诺如病毒(Norovirus,NoV)感染情况及其分子病原学特征,为NoV预防控制和疫苗研发提供参考。方法收集德阳市2018年3起AGE暴发疫情的疑似病例及健康人标本共63份。采用实时荧光RT-PCR检测NoV核酸,阳性标本用RT-PCR扩增聚合酶(RNA-dependent RNA polymerase,RdRp)区和衣壳蛋白(capsid protein)VP1区并测序分析。结果NoV实时荧光RT-PCR结果显示3起AGE暴发疫情均由GII组NoV感染引起,检测阳性率为33.3%(21/63),其中病例标本检出率为95%(19/20),健康人标本检出率为4.7%(2/43)。测序分析表明,2起疫情由GII.17[P17]型NoV引起,与2014年以来流行的GII.17变异株高度同源;1起疫情由GII.2[P16]型NoV引起,与2016年以来流行的GII.2[P16]重组株高度同源。结论2018年德阳市至少存在2种以上不同型别的NoV同时流行。Objective To investigate the genotype and genetic characteristics of the pathogens associated with the epidemic outbreak of acute gastroenteritis(AGE)in Deyang city,Sichuan province,and to provide perspectives on disease prevention and vaccine development.Methods Norovirus(NoV)nucleic acid in the sixty-three samples collected from three AGE outbreaks were detected by Real-time RT-PCR.The genes of RNA-dependent RNA polymerase(RdRp)and capsid protein(VP1)of the positive samples were amplified by conventional RT-PCR and sequenced and made phylogenetic analysis.Results Three AGE outbreaks were all caused by NoV,and twenty-one samples were positive for NoV GII.Two of the outbreaks were caused by GII.17[P17]NoV and shared high similarities with global reference sequences from 2014.One of the outbreaks was caused by GII.2[P16]NoV and shared high similarities with global reference sequences from 2016.Conclusions The three AGE outbreaks in Deyang city in 2018 were caused by two different NoV genotypes.

关 键 词：诺如病毒 胃肠炎 暴发 系统进化分析 

分 类 号：R512.5[医药卫生—内科学] R440[医药卫生—临床医学]