基于^1H-NMR植物代谢组学技术分析青海产区枸杞子的化学特征  

Analysis on Chemical Characteristics of Lycii Fructus in Qinghai Province Based on ^1H-NMR Plant Metabolomics

作　　者：赵光跃 魏玉海 苏姗姗 喇红玲 严培瑛 李彩明 李震宇[3] ZHAO Guang-yue;WEI Yu-hai;SU Shan-shan;LA Hong-ling;YAN Pei-ying;LI Cai-ming;LI Zhen-yu(Key Laboratory of Food Safety Research in Qinghai Province,Technical Center of Xining Customs,Xining 810001,China;School of Life Sciences,Qinghai Normal University,Xining 810001,China;Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China)

机构地区：[1]西宁海关技术中心青海省食品安全研究重点实验室,西宁810001 [2]青海师范大学生命科学学院,西宁810001 [3]山西大学中医药现代研究中心,太原030006

出　　处：《中国实验方剂学杂志》2020年第17期95-103,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“十三五”重点研发计划项目(2017YFF021130304);青海科技计划项目(2017-ZJ-762)。

摘　　要：目的:采用核磁共振氢谱(^1H-NMR)植物代谢组学技术比较青海产区枸杞子与其他产区(宁夏、甘肃、新疆、内蒙古)枸杞子的化学成分差异。方法:收集5个产区共97份枸杞子样本,其中青海61个样本,采用50%甲醇提取,检测^1H-NMR图谱,结合多元统计分析,对比青海产区枸杞子与其他产区枸杞子的化学差异性,并对各产区样本的枸杞多糖进行含量测定(以无水葡萄糖计),检测波长490 nm。结果:枸杞子的^1H-NMR图谱共检测到32个化学成分,多元统计分析表明青海产区枸杞子与其他产区样本相比,无明显分离趋势;青海产区枸杞子与宁夏产区相比,以及青海省6个不同地区的枸杞子相比,重叠样品较多,均不能显著分开。相似度结果表明,大多数样品的相似度>0.85;化合物的单变量分析结果显示,除了蔗糖、葡萄糖、脯氨酸等个别代谢物在各产区样本中存在显著差异外,其余代谢物在各产区样品中的含量分布基本一致。青海与其他产区样本中枸杞多糖含量无显著性差异,且枸杞多糖含量与^1H-NMR指认的小分子化合物的相关系数处于-0.2~0.4。结论:采用^1HNMR植物代谢组学技术从整体化学组成上分析了青海产区枸杞子的化学特征,并结合枸杞多糖含量测定,显示青海产区枸杞子与其他产区枸杞子的化学差异较小。建立的基于^1H-NMR的枸杞子质量评价方法可为其质控水平提升及种植产区选择提供科学依据。Objective:The chemical differences of Lycii Fructus samples from Qinghai,Ningxia,Gansu,Xinjiang and Inner Mongolia provinces were compared based on proton nuclear magnetic resonance(^1HNMR)plant metabolomics.Method:A total of 97 Lycii Fructus samples from five provinces were collected,including 61 samples in Qinghai,and extracted by 50%methanol for detecting.^1H-NMR spectra were obtained and compared by multivariate statistical analysis for investigating the chemical differences of samples from Qinghai and other production areas.And the content of Lycii Fructus polysaccharides in all samples was determined with the wavelength of 490 nm(calculated by anhydrous glucose).Result:A total of 32 chemical components were detected in the Lycii Fructus extract by^1H-NMR.The multivariate statistical analysis revealed that there was no significant difference among the samples from five provinces.The difference between Lycii Fructus from Qinghai and Ningxia,as well as the samples among the six regions of Qinghai province were relatively small.The similarity values of the majority of samples were>0.85.Univariate analysis showed that no significant difference was observed for the most metabolites in Lycii Fructus collected from five provinces,except for sucrose,glucose,proline and so on.There was no significant difference in the content of Lycii Fructus polysaccharides between Qinghai and other provinces.And the correlation coefficient between the content of Lycii Fructus polysaccharides and the small molecular compounds identified by^1H-NMR was-0.2-0.4.Conclusion:In this study,chemical characteristics of Lycii Fructus in Qinghai province are analyzed from the holistic view by^1H-NMR plant metabolomics,in combination of polysaccharide determination,and the results show that there is no significant difference between samples from Qinghai and other four provinces.The quality evaluation method based on^1H-NMR established in this study can provide scientific basis for improving quality control level and selecting planting areas of

关 键 词：枸杞子 核磁共振氢谱(^1H-NMR) 化学差异 代谢组学 相似度 多糖 Pearson相关性 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学]