基于液质联用技术和植物代谢组学的甘草炮制品化学成分差异性分析  

Analysis of Chemical Variability on Different Processed Products of Glycyrrhiza uralensis Dry Roots and Rhizomes Based on UPLC-Q-TOF-MS^E and Plant Metabolomics

作　　者：文旺 李莉 李德坤[2,3] 张磊[2,3] 周大铮[3] 杨悦武 杨建会 余伯阳 鞠爱春 WEN Wang;LI Li;LI De-kun;ZHANG Lei;ZHOU Da-zheng;YANG Yue-wu;YANG Jian-Hui;YU Bo-yang;JU Ai-chun(School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China;Tianjin Tasly Pride Pharmaceutical Co.Ltd.,Tianjin 300410,China;Tasly Holding Group Co.Ltd.,Tianjin 300410,China)

机构地区：[1]中国药科大学中药学院,南京211198 [2]天津天士力之骄药业有限公司,天津300410 [3]天士力控股集团有限公司,天津300410

出　　处：《中国实验方剂学杂志》2020年第17期104-110,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市科技重大专项与工程项目(18ZXXYSY00020)。

摘　　要：目的:识别并鉴定不同甘草炮制品的质量差异标志物。方法:运用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS^E)技术,通过负离子检测模式,采集各甘草炮制品成分高精度质荷比及离子响应强度信息;利用主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)对预处理后的数据集进行分析,快速搜寻出不同甘草炮制品间的差异性成分,依据相对分子质量、碎片离子、质谱数据库和相关文献对差异性成分进行鉴定,研究甘草炮制前后成分迁移情况。结果:从甘草片、清炒甘草、蜜炙甘草3组炮制品中共识别10个质量差异标志物,主要为甘草苷和甘草酸的衍生物,其中6’’-Oacetylliquiritin apioside,6’’-O-acetylliquiritin apioside异构体,6’’-O-acetylliquiritin,芒柄花黄素和11-脱氧-18β-甘草亭酸在甘草片中含量最高;6’’-O-acetylisoliquiritin apioside,6’’-O-acetylisoliquiritin,异甘草苷和单葡萄糖醛酸甘草次酸在清炒甘草中含量最高;甘草苷在蜜炙甘草中含量最低。结论:甘草片、清炒甘草和蜜炙甘草存在一定的化学成分差异,可为甘草炮制品的质量控制和药效研究奠定物质基础。Objective:To identify the quality differential markers of different processed products of Glycyrrhiza uralensis dry roots and rhizomes.Method:Ultra-high performance liquid chromatographyquadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS^E)was used to collect high-precision masscharge ratio and ion response strength information of the components in G.uralensis dry roots and rhizomes before and after processing by negative ion mode.The data set collected after pretreatment was analyzed with principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)to quickly search the differential components in different processed products of G.uralensis dry roots and rhizomes.Differential components were identified according to the relative molecular weight,fragment ion,mass spectrum database and related literature information,then the migration of components before and after processing was studied.Result:A total of 10 quality differential markers were searched from raw products,roasted products and honey-roasted products of G.uralensis dry roots and rhizomes,mainly derivatives of liquiritin and glycyrrhizic acid.Among them,the contents of 6’’-O-acetylliquiritin apioside,6’’-O-acetylliquiritin apioside isomer,6’’-O-acetylliquiritin,formononetin and 11-deoxo-18β-glycyrrhetic acid were the highest in the raw products,the contents of 6’’-O-acetylisoliquiritin apioside,6’’-O-acetylisoliquiritin,isoliquiritin and glycyrrhetic acid 3-O-glucuronide were the highest in the roasted products,the content of liquiritin was the lowest in the honey-roasted products.Conclusion:There are some chemical differences among the three products.This study can provide material basis for the quality control and pharmacodynamic research of processed products of G.uralensis dry roots and rhizomes.

关 键 词：甘草 炮制品 植物代谢组学 质量差异标志物 超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS^E) 化学成分 甘草苷 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学]