XIAP基因对食管癌细胞黏附、侵袭和迁移能力的影响  

Effects of XIAP gene on adhesion,invasion and migration of esophageal cancer cells

作　　者：杜芳 侯晓东[2] Du Fang;Hou Xiaodong(Department of Hematology and Oncology,988 Hospital of Joint Logistics Support Force,Henan Zhengzhou 450042,China;Department of Ultrasound Medicine,the First Affiliated Hospital of Henan University,Henan Kaifeng 475100,China)

机构地区：[1]联勤保障部队第988医院血液肿瘤科,河南郑州450042 [2]河南大学第一附属医院超声医学科,河南开封475100

出　　处：《现代肿瘤医学》2020年第17期2939-2942,共4页Journal of Modern Oncology

摘　　要：目的:探讨沉默X连锁凋亡抑制蛋白(XIAP)基因表达对食管癌细胞黏附、侵袭和迁移能力的影响及机制。方法:参照LipofectamineTM2000说明将设计合成的XIAP特异性siRNA(si-XIAP)转染人食管癌EC9706细胞,将转染阴性对照siRNA(NC组)和仅加入脂质体的空白细胞作为两个对照组,siRNA转染48 h,通过Western blotting检测XIAP、AKT、p-AKT、E-cadherin和MMP-2蛋白表达;通过MTT法、Transwell法分别检测细胞黏附、侵袭和迁移能力;MTT法检测转染siRNA后24 h、48 h和72 h的细胞增殖。结果:XIAP特异性siRNA转染后,EC9706细胞XIAP表达明显降低,与空白组比较差异有统计学意义(P<0.05)。与空白组比较,si-XIAP组细胞增殖明显降低,黏附率明显升高,侵袭和迁移能力明显降低,p-AKT和MMP-2表达明显降低,E-cadherin表达明显升高(P<0.05)。结论:沉默XIAP基因可增强食管癌细胞黏附率,抑制侵袭和迁移能力,机制与抑制PI3K/AKT信号通路有关。Objective:To investigate the effect and mechanism of XIAP gene silencing on adhesion,invasion and migration of esophageal carcinoma cells.Methods:According to LipofectamineTM2000,XIAP-specific siRNA(si-XIAP)was transfected into human esophageal carcinoma EC9706 cells.Negative control siRNA(NC group)and Blank cells added only to liposomes were used as control group.siRNA was transfected for 48 hours.XIAP,AKT,p-AKT,E-cadherin and MMP-2 protein expression were detected by Western blotting.Cell adhesion,invasion and migration were detected by MTT and Transwell methods,and cell proliferation was detected by MTT at 24,48 and 72 hours after transfection of siRNA.Results:After XIAP specific siRNA was transfected into EC9706 cells,the expression of XIAP in EC9706 cells was significantly lower than that in control group(P<0.05).Compared with si-XIAP group and the blank group,cell proliferation decreased significantly,adhesion rate increased significantly,invasion and migration ability decreased significantly,p-AKT and MMP-2 expression decreased significantly,and E-cadherin expression increased significantly(P<0.05).Conclusion:Silencing XIAP gene can enhance adhesion and inhibit invasion and migration of esophageal cancer cells,which may be related to the inhibition of PI3 K/AKT signaling pathway.

关 键 词：食管癌 XIAP基因 黏附 侵袭迁移 PI3K/AKT信号通路 

分 类 号：R735.1[医药卫生—肿瘤]