单次热应激诱导小鼠睾丸间质细胞乙醛脱氢酶蛋白表达的变化  

Change of ALDH2 expression in mouse testicular mesenchymal cells induced by single heat stress

作　　者：田洪成 孟夏 马鹤[1] 王会君[1] 李清春[1] 王雁林[1] TIAN Hongcheng;MENG Xia;MA He;WANG Huijun;LI Qingchun;WANG Yanlin(Department of Reproductive Medicine,Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China)

机构地区：[1]滨州医学院附属医院生殖医学科,山东滨州256603

出　　处：《滨州医学院学报》2020年第4期245-248,共4页Journal of Binzhou Medical University

基　　金：滨州医学院科技计划(BY2017KJ07,BY2016KJ51)。

摘　　要：目的探讨ALDH2在单次热应激致小鼠睾丸功能可逆性变化中的作用。方法C57雄性小鼠18只,随机分成对照组和热处理组,热处理组按处理后1、7、14 d分成3个亚组(n=3),每组设立对照组(n=3),热应激组和对照组小鼠下腹部分别置于43℃和25℃水浴中干预15 min,于处理后1、7、14 d取睾丸组织。免疫组化染色检测ALDH2蛋白定位及表达,Western印迹检测ALDH2蛋白表达水平。结果免疫组化显示,ALDH2蛋白在睾丸间质细胞中表达。Western印迹检测显示,与对照组相比,热应激后第1天,ALDH2蛋白表达无明显改变;热应激后第7天、14天较对照组及热应激第1天比较,ALDH2蛋白表达明显增多(P<0.01);热应激第7天与热应激第14天,ALDH2蛋白表达无明显改变。结论小鼠在热应激处理后的第7、14天,ALDH2蛋白表达量在睾丸组织中显著增加。Objective To investigate the role of ALDH2 in the reversibility of mouse testicular reproductive function impaired by single heat stress.Methods Eighteen C57 male mice were randomly divided into the heat stress group and the control group and immersed in water at 43℃and 25℃,respectively,for 15 minutes.The heat treatment group was divided into 3 subgroups(n=3)on day 1,7 and 14 after treatment,and a control group(n=3)was set in each group.At 1,7,and 14 days of heat exposure,all the mice were sacrificed and their testis tissues collected for measuring the expression level of the ALDH2 protein by immunohistochemistry and Western blot.Results Immunohistochemistry showed that ALDH2 protein was expressed in Leydig's cells.Western blot showed that there was no significant difference in ALDH2 protein expression on the first day after thermal stress compared with the control group.Compared with the control group and the first day of thermal stress,ALDH2 protein expression was significantly increased on day 7 and 14 after thermal stress(P<0.01).There was no significant difference in ALDH2 protein expression between day 7 and Day 14 of thermal stress.Conclusion The expression of ALDH2 protein increased significantly in 7 days and 14 days after exposure.

关 键 词：单次热应激 小鼠 睾丸间质细胞 ALDH2 

分 类 号：R169.41[医药卫生—公共卫生与预防医学]