Brassinosteroids regulate outer ovule integument growth in part via the control of INNER NO OUTER by BRASSINOZOLE‐RESISTANT family transcription factors  

作　　者：Dandan Jia Lian-Ge Chen Guimin Yin Xiaorui Yang Zhihua Gao Yi Guo Yu Sun Wenqiang Tang 

机构地区：[1]Ministry of Education Key Laboratory of Molecular and Cellular Biology,Hebei Collaboration Innovation Center for Cell Signaling,Hebei KeyLaboratory of Molecular and Cellular Biology,College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,China [2]College of Bioscience&Bioengineering,Hebei University of Science and Technology,Shijiazhuang 050018,China

出　　处：《Journal of Integrative Plant Biology》2020年第8期1093-1111,共19页植物学报（英文版）

基　　金：This study wassupported by grants from the National Natural Science Foundation of China(91417313 and 2014CB943404 to W.T.);Department of Education of Hebei Province(LJRC015 to W.T.);the“one hundred talents project”of Hebei province(E2013100004 to Y.S.)。

摘　　要：Brassinosteroids(BRs)play important roles in regulating plant reproductive processes.BR signaling or BR biosynthesis null mutants do not produce seeds under natural conditions,but the molecular mechanism underlying this infertility is poorly understood.In this study,we report that outer integument growth and embryo sac development were impaired in the ovules of the Arabidopsis thaliana BR receptor null mutant bri1-116.Gene expression and RNA-seq analyses showed that the expression of INNER NO OUTER(INO),an essential regulator of outer integument growth,was significantly reduced in the bri1-116 mutant.Increased INO expression due to overexpression or increased transcriptional activity of BRASSINAZOLE-RESISTANT 1(BZR1)in the mutant alleviated the outer integument growth defect in bri1-116 ovules,suggesting that BRs regulate outer integument growth partially via BZR1-mediated transcriptional regulation of INO.Meanwhile,INO expression in bzr-h,a null mutant for all BZR1 family genes,was barely detectable;and the outer integument of bzr-h ovules had much more severe growth defects than those of the bri1-116 mutant.Together,our findings establish a new role for BRs in regulating ovule development and suggest that BZR1 family transcription factors might regulate outer integument growth through both BRI1-dependent and BRI1-independent pathways.

关 键 词：IMPAIRED outer STEROIDS 

分 类 号：Q943.2[生物学—植物学]