激光散斑成像法监测格列本脲对小鼠SAH后脑微循环的影响  

Effects of glibenclamide on cerebral microcirculation after subarachnoid hemorrhage in mice

作　　者：刘鹏 唐跃 佘德源 马廉亭 潘力 何川[1] LIU Peng;TANG Yue;SHE De-yuan;MA Lian-ting;PAN Li;HE Chuan(Department of Neurosurgery,Xuanwu Hospital,Capital Medical University,Beijing 100053,China;Department of Neurosurgery,General Hospital of Central Theater Command,PLA,Wuhan 430070,China)

机构地区：[1]首都医科大学宣武医院神经外科,北京100053 [2]中国人民解放军中部战区总医院神经外科,武汉430070

出　　处：《中国临床神经外科杂志》2020年第8期538-541,共4页Chinese Journal of Clinical Neurosurgery

基　　金：国家自然科学基金(81870945);中国博士后科学基金(2018M641411);湖北省卫生与计划生育委员会科研联合项目(WJ2018H0065)。

摘　　要：目的探讨格列本脲对小鼠蛛网膜下腔出血(SAH)后脑微循环的影响。方法将28只雄性C57小鼠随机分为对照组(n=6)、SAH组(n=6)、溶媒组(n=6)和格列本脲组(n=10)。视交叉前池注入自体非抗凝尾动脉血60μl建立SAH模型。小鼠SAH后5 min内,格列本脲组腹腔注射格列本脲(25μg/kg),溶媒组腹腔注射等体积二甲基亚砜;SAH组未进行任何治疗。造模后12 h,应用Garcia量表评分评估神经功能;造模前后使用激光散斑血流成像监测脑皮层灌注以及体感刺激下感觉皮层血流响应幅度。结果造模后12 h,SAH组、溶媒组神经功能评分明显下降(P<0.05),脑感觉皮层灌注明显下降(P<0.05),体感刺激下感觉皮层血流响应幅度明显下降(P<0.05)。应用格列本脲后,小鼠神经功能评分明显增高(P<0.05),脑感觉皮层血流响应幅度和感觉皮层灌注明显改善(P<0.05)。结论格列本脲可以改善小鼠SAH后神经功能,可能与改善脑微循环有关。Objective To explore the effect of glibenclamide on the cerebral microcirculation after subarachnoid hemorrhage(SAH)in mice.Methods Twenty-eight male C57 mice were randomly divided into control group(n=6),SAH group(n=6),vehicle group(n=6)and glibenclamide group(n=10).The SAH model was established by injecting 60μl of autologous non-anticoagulated tail artery blood into the anterior cisterna.Within 5 minutes after SAH,the mice in the glibenclamide group were intraperitoneally injected with glibenclamide(25μg/kg),the mice in the vehicle group were intraperitoneally injected with the same volume of dimethyl sulfoxide,and the mice in the SAH group received no treatment.Twelve hours after SAH,the Garcia scale was used to evaluate neurological function;laser speckle blood flow imaging was used to monitor cerebral cortex perfusion and the response amplitude of sensory cortex blood flow under somatosensory stimulation before and after SAH.Results After SAH,the neurological function score was significantly decreased(P<0.05),the cerebral sensory cortex perfusion was significantly decreased(P<0.05),and the sensory cortex blood flow response amplitude was significantly decreased under somatosensory stimulation(P<0.05).After the application of glibenclamide,the neurological score was significantly increased(P<0.05),and the blood flow response amplitude and perfusion of the sensory cortex of the brain were significantly improved(P<0.05).Conclusion Glibenclamide can improve the neurological function of mice after SAH,which may be related to the improvement of cerebral microcirculation.

关 键 词：蛛网膜下腔出血 感觉皮层灌注 血流响应 格列本脲 激光散斑成像 小鼠 

分 类 号：R743.9[医药卫生—神经病学与精神病学]