芜菁Br14-3-3的克隆及其在非生物胁迫下的表达分析  

Cloning and Expression Under Abiotic Stress of Br14-3-3 in Brassica rapa subsp.rapifera

作　　者：王若男 李菊 苗鸿钰 闫海芳[1] WANG Ruonan;LI Ju;MIAO Hongyu;YAN Haifang(College of Life Sciences,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《园艺学报》2020年第7期1301-1311,共11页Acta Horticulturae Sinica

基　　金：黑龙江省自然科学基金面上项目(C2017001);东北林业大学大学生创新训练项目(201910225335)。

摘　　要：克隆了‘津田芜菁’(Brassica rapa subsp.rapifera‘Tsuda’)通用调节因子14-3-3基因cDNA序列,命名为Br14-3-3(GenBank登录号为MK896872)。该基因全长1113 bp,开放阅读框全长774 bp,编码含有257个氨基酸的多肽。荧光定量PCR分析Br14-3-3在‘津田芜菁’不同组织中及其在温度、脱水、渗透、ABA和无机盐等非生物胁迫下幼苗中的表达,结果表明该基因在‘津田芜菁’的花中表达量最高,幼苗中次之;低温抑制了Br14-3-3的表达,其他非生物胁迫可诱导该基因表达,暗示Br14-3-3在非生物胁迫应答中发挥功能。In this study,we cloned the general regulatory factor 14-3-3 gene from Brassica rapa subsp.rapifera’Tsuda’,which was named Br14-3-3 with the accession number of MK896872 in the NCBI GenBank.The full cDNA sequence of the Br14-3-3 gene was 1113 bp,cotaining an open reading frame of 774 bp encoding a protein of 257 amino acids.We determined the expression of the Br14-3-3 gene in different tissues and under different abiotic stress conditions,such as extreme temperature,dehydration,adverse osmosis,abscisic acid(ABA)and salt stress by quantitative-PCR analysis.The results demonstrated that the highest expression levels of the Br14-3-3 gene could be reached in the flower of Brassica rapa subsp.rapifera’Tsuda’,followed by the seedling.The expression of the Br14-3-3 gene was upregulated under aforementioned stress conditions except for low temperature,suggesting that the Br14-3-3 gene may play a role in response of abiotic stresses.

关 键 词：津田芜菁 14-3-3 基因克隆 胁迫 表达 

分 类 号：S631.3[农业科学—蔬菜学]