大肠不同部位恶性上皮肿瘤中错配修复蛋白表达及意义  

Expression and Significance of Mismatch Repair Protein in Malignant Epithelial Tumors in Different Parts of the Large Intestine

作　　者：段运动[1] 代俊利[1] DUAN Yongdong;DAI Junli(Chengde Central Hospital, Hebei Chengde 067000, China)

机构地区：[1]河北省承德市中心医院重症医学科,河北承德067000

出　　处：《河北医学》2020年第8期1321-1324,共4页Hebei Medicine

基　　金：河北省2018承德市科学技术研究与发展计划项目,(编号:201804A078)。

摘　　要：目的:探讨大肠中不同部位大肠癌组织中错配修复蛋白(Mismatch repair,MMR)的表达及MMR在大肠癌中的表达意义。方法:采用免疫组化方法(SP法)标记大肠癌组织中错配修复蛋白(MSH1、MSH2、MSH6及PMS2)的表达并分析其意义,同时分析MMR蛋白在不同部位大肠癌中的表达意义。MSH1、MSH2、MSH6及PMS2四种蛋白中的1种及以上表达缺失判定为错配修复基因缺陷(dMMR),全部表达判定为错配基因完整(pMMR)。结果:在168例大肠癌组织中,pMMR为141例(83.93%),dMMR 27例(16.07%),78例癌旁正常大肠黏膜组织MMR蛋白表达缺失组(dMMR)56例(71.79%),MMR蛋白无缺失组(pMMR)为22例(28.21%)(P<0.05)。同时分析发现,在大肠癌组织中,dMMR与大肠癌发病部位有相关性,在47例右半结肠癌患者中,dMMR共有19例(40.42%),左半结肠癌92例当中,dMMR共有5例(5.43%),在29例直肠癌组织中,dMMR有3例(10.34%)。而且dMMR与大肠癌临床病理特征有一定关系,经分析有淋巴结转移组,侵透肌层组dMMR要明显低于无淋巴结转移组、侵犯肌层组(P<0.05)。结论:错配修复蛋白在大肠癌中表达高于正常肠黏膜,而且错配修复蛋白在不同部位大肠癌中表达不同,不同错配修复蛋白状态与大肠癌的进展有一定相关性,而且提示具有错配修复蛋白缺失的大肠癌预后可能会更好。Objective:To investigate the expression of mismatch repair protein(MMR)in colorectal cancer tissues in different parts of the large intestine and the significance of MMR expression in colorectal cancer.Methods:The expression and significance of mismatch repair proteins(msh1,MSH2,MSH6,and PMS2)in colorectal cancer tissues were analyzed by immunohistochemical method(SP method),and the expression significance of MMR protein in different parts of colorectal cancer was analyzed.Deletion of one or more of msh1,MSH2,MSH6 and PMS2 proteins was determined as mismatch repair gene defect(dmmr),and all expression levels were determined as mismatch repair gene integrity(PMMR).Results:In 168 cases of colorectal cancer,there were 141 cases(83.93%)of PMMR,27 cases(16.07%)of dmmr,56 cases(71.79%)of MMR protein expression loss group(dmmr)and 22 cases(28.21%)of MMR protein non deletion group(PMMR)in 78 cases of normal colorectal mucosa adjacent to carcinoma(P<0.05).In 47 cases of right colon cancer,there were 19 cases(40.42%)of dmmr,5 cases(5.43%)of left colon cancer,3 cases(10.34%)of 29 cases of rectal cancer.Furthermore,there was a certain relationship between dmmr and clinicopathological features of colorectal cancer.The dmmr in the group with lymph node metastasis and invasion into the muscular layer was significantly lower than that in the group without lymph node metastasis and the group with invasion of muscle layer(P<0.05).Conclusion:The expression of mismatch repair protein in colorectal cancer is higher than that in normal intestinal mucosa,and the expression of mismatch repair protein is different in different parts of colorectal cancer.Different status of mismatch repair protein is related to the progress of colorectal cancer,and suggests that the prognosis of colorectal cancer with mismatch repair protein deficiency may be better.

关 键 词：大肠癌 错配修复蛋白 部位 

分 类 号：R73[医药卫生—肿瘤]