三种常用溶剂对98种农残检测结果稳定性的影响  

Study on the stability of 98 pesticide standards in three common solvents

作　　者：张伟彪 顾亚萍 曾定玲 王瑞 林慧纯 尹义捐 方长发[3] ZHANG Weibiao;GU Yaping;ZENG Dingling;WANG Rui;LIN Huichun;YIN Yijuan;FANG Changfa(Shenzhen Centre of Inspection and Testing for Agricultural Products,Shenzhen 518040,China;Key Laboratory of Supervision for Edible Agricultural Products,Administration for Market Regulation of Guangdong Province,Shenzhen 518040,China;School of Applied Chemistry and Biological Technology,Shenzhen Polytechnic,Shenzhen 518055,China)

机构地区：[1]深圳市农产品质量安全检验检测中心,广东深圳518040 [2]广东省市场监督管理局食用农产品监管重点实验室,广东深圳518040 [3]深圳职业技术学院应用化学与生物技术学院,广东深圳518055

出　　处：《食品与发酵工业》2020年第16期236-242,共7页Food and Fermentation Industries

摘　　要：研究QuEChERS-液相色谱串联四极杆质谱(liquid chromatography tandem quadrupole mass spectrometry,LC-MS-MS)方法中,乙腈、乙腈+水(体积比1∶1)和甲醇+水(体积比1∶1) 3种常用的溶剂对98种农药标准物质和2种加标基质稳定性的影响。标准溶液(浓度为0.080μg/mL) 11 h的连续测定结果显示,甲醇+水(体积比1∶1)中标准物质峰面积长时间的稳定性最好,乙腈+水(体积比1∶1)次之,乙腈最差。使用甲醇+水(体积比1∶1)配制标准物质时,长期不稳定的农药仅为四螨嗪、氟苯脲、杀扑磷和异菌脲。对韭菜和橙子2种加标基质放置3 h的稳定性研究结果表明,橙子基质中农药测定结果在3种溶剂中稳定性均优于韭菜,而以甲醇+水(体积比1∶1)作为溶剂对于检测结果的稳定性贡献更大。The stability of 98 pesticide standards and two matrix standards in three commonly used solvents,namely acetonitrile,acetonitrile/water(1∶1),and methanol/water(1∶1),was tested using QuEChERS-LC-MS-MS. The continuous measurement results of the standards for 11 hours showed that the peak area of the standards in methanol/water showed relative long-term stability than that in acetonitrile/water;while the stability in acetonitrile was poor. When methanol/water was used to prepare the standards,tetrabenazine,flufenuron,fenflufen,and isocarbazone were to be unstable. The unstable pesticides in methanol/water were tetramazine,flubenzuron,fenflufen,and allazurea. The stability results of the two matrix standards for 3 hours in leeks and oranges indicated that pesticide stability in the orange matrix was better,and methanol/water contributed more to the stability of the test results.

关 键 词：QuEChERS法 液相色谱串联四极杆质谱仪 基质效应 标样稳定性 溶剂稳定性 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]