浙八味煎剂治疗非酒精性脂肪性肝纤维化的实验研究  

Experimental study on Eight Zhes Decoction in the treatm ent of nonalcoholic fatty liver fibrosis

作　　者：周玉平 吕雪幼 温晋锋[1,2] 杨萍 周飞[4] 唐春兰 ZHOU Yu-ping;LYU Xue-you;WEN Jin-feng;YANG Ping;ZHOU Fei;TANG Chun-lan(Department of Gastroenterology and Hepatology,the Affiliated Hospital of Medical School of Ningbo University,Ningbo,Zhejiang 315020,China)

机构地区：[1]宁波大学医学院附属医院消化内科,浙江宁波315020 [2]宁波大学消化疾病研究所,浙江宁波315020 [3]宁波卫生职业技术学院护理学院,浙江宁波315100 [4]宁波大学医学院实验动物中心,浙江宁波315211 [5]宁波大学医学院预防医学系

出　　处：《中华全科医学》2020年第10期1641-1644,1674,共5页Chinese Journal of General Practice

基　　金：浙江省中医药科技计划(2019ZB114);宁波市自然科学基金(2019A610217)。

摘　　要：目的研究临床经验方"浙八味煎剂"对非酒精性脂肪性肝纤维化(NASF)小鼠模型的治疗作用。方法C57 BL/6J雄性小鼠,采用随机数字表法随机分为4组,正常组.模型组、浙八味煎剂高、低剂量组,每组8只。采用课题组改良的蛋氨酸胆碱缺乏(MCD)结合反复低剂量腹腔注射CCI.制备动物模型。造模第3周首日开始予浙八味煎剂进行灌胃,6周末处死动物采血和肝组织,检测各组血清肝功能(ALT、AST、TBil)水平,血清和肝脏甘油三酯(TG)含量,观察各组肝组织HE染色、油红p染色、天狼猩红病理染色情况,采用免疫组化方法检测肝组织a-平滑肌肌动蛋白(x-SMA)表达情况。采用单因素方差分析和LSD检验方法进行数据分析。结果相对正常组,模型组小鼠血清ALT.AST.TBil活性及肝脏TG含量明显升高(均P<0.01);肝组织出现广泛炎症反应,油红0阳性染色、Masson胶原染色及aSMA蛋白阳性表达面积显著增加(均P<0.01);浙八味煎剂高剂量组显著降低血清ALT、AST、TBil活性及肝脏TG含量(均P<0.01).显著减少肝组织油红0阳性染色、Masson胶原染色及a-SMA蛋白阳性表达面积(均P<0.01)。结论“浙八味煎剂”对非酒精性脂肪性肝纤维化模型小鼠具有良好的治疗作用,抑制星状细胞活化可能是其部分机制。Objective To study the therapeutie efect of Eight Zhes Decoction(EZD)on nonalcoholie ftty liver fibrosis(NASF)in mice model.Methods Thirty-two male C57BL/6 mice were randomly divided into control group,model group,EZD high dose group and EZD low dose group.The control group mice were fed with methionine-choline supple-mented diet(MCS),while the other groups with methionine and choline deficient(MCD)diet and repeated injection of carbon tetrachloride(CCI4)once a week.On the first day of the third week,the EZD group were intragastric administra-ted by EZD,and at the end of the sixth week,all the experimental mice were sacrificed,the serums and liver were col-lected.The histological analysis was conducted by HE staining,oil red 0 staining and Sirius red staining.The hepatic al-pha smooth muscle actin(a:SMA)protein expression was detected by immunohistochemistry.In addition,serum liver function and liver triglyceride levels were measured.Results Compared with the control group,the activities of ALT,AST and TBil in serum and the triglyceride content in liver of model group mice were significantly inereased(all P<0.01),which were signifcant reduced in the EZD group(all P<0.01).Compared with the control group,liver tissue in model group showed obrious inflammation and increased the areas of oil red O-positive staining,Masson collagen staining(all P<0.01),which were significant reduced in the EZD group(all P<0.01).In addition,model group expressed higher level of a-SMA protein(P<0.01),which were reduced in the EZD group(P<0.01).Conclusion Eight Zhes Decoction could prevent liver fibrosis in mice model of NASF.The underlying mechanism might partially lie in inhibiting the activation of hepatic sellatle cells and ollagen production.

关 键 词：浙八味煎剂 道地药材 非酒精性脂肪性肝纤维化 动物模型 

分 类 号：R575.2[医药卫生—消化系统] R-332[医药卫生—内科学]