透明质酸及其合成酶在炎症牙髓组织中的表达  

Expression of hyaluronic acid and hyaluronic acid synthases in dental pulp tissue under inflammation

作　　者：陈蔚婷 蒋备战[1] CHEN Weiting;JIANG Beizhan(Department of Pediatric Dentistry,School&Hospital of Stomatology,Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Shanghai 200072,China)

机构地区：[1]同济大学口腔医学院儿童口腔医学教研室,上海牙组织修复与再生工程技术研究中心,上海200072

出　　处：《口腔医学》2020年第7期606-611,共6页Stomatology

基　　金：上海市科学技术委员会计划(18411969500)。

摘　　要：目的检测透明质酸(HA)及其合成酶(HAS1、HAS2、HAS3)在炎症牙髓组织中的表达,为研究其在牙髓组织炎症调控过程中的作用提供依据。方法牙髓组织来自健康患者完全埋伏阻生及萌出后因深龋致牙髓炎症的第三磨牙,采用免疫组织化学(IHC)及免疫荧光(IF)染色法检测所取牙髓组织中HA、HAS1、HAS2、HAS3及肿瘤坏死因子α(TNFα)的表达,用实时定量聚合酶链式反应(quantitative real-time polymerase chain reaction,qRT-PCR)对HAS1、HAS2、HAS3及相关炎症因子IL-6、IL-8、TNFα在健康及炎症牙髓组织中的表达情况进行定量检测。结果IHC及IF染色结果表明,HA、HAS2、HAS3和TNFα在炎症牙髓组织中的表达比健康牙髓组织中明显;HA、HAS2、HAS3分别和TNFα之间存在共表达;且炎症牙髓组织中HAS2的荧光染色信号比HAS3更强;而HAS1的荧光信号在健康及炎症牙髓组织中均较弱。qRT-PCR结果提示炎症牙髓组织中IL-6、IL-8、TNFα(PIL-6,IL-8,TNFα<0.01)表达较正常牙髓组织强,同时HAS2、HAS3(PHAS2,HAS3<0.01)的表达亦随之显著升高。结论HA以及HAS2、HAS3在炎症牙髓组织中的表达明显上调,这提示透明质酸可能参与了对牙髓组织炎症状态的调控。Objective To detect the expression of the hyaluronic acid(HA)and hyaluronic acid synthases(HAS1,HAS2,HAS3)in the inflamed pulp tissue,and provide reference for their roles in the process of pulp inflammation.Methods Pulp tissues from the fully impacted mandibular third molars and the erupted third molars in a state of inflammation caused by deep caries in healthy patients were collected.The expressions of HA,HAS1,HAS2,HAS3 and TNFα in the collected dental pulp were detected by immunohistochemical staining and immunofluorescence staining.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of HAS1,HAS2,HAS3 and the related inflammatory cytokines IL-6,IL-8,TNFαin the collected samples.Results The IHC and IF results showed that in the inflamed pulp tissue,the expressions of HA,HAS2 and HAS3,which were co-stained with TNFα,were stronger compared with in healthy pulp tissue.The expression of HAS2 in the inflamed pulp tissue was much stronger than that of HAS3,and the expression of HAS1 was weaker in healthy and inflamed pulp tissue.The results of qRT-PCR indicated that in the inflamed pulp,the expression levels of IL-6,IL-8,TNFα(PIL-6,IL-8,TNFα<0.01)were stronger than in the normal pulp tissue,and the expressions of HAS2 and HAS3(PHAS2,HAS3<0.01)significantly increased compared with in the normal pulp.Conclusion The expressions of HA,HAS2 and HAS3 are significantly up-regulated in the dental pulp tissue under inflammation,which suggests that HA may be involved in regulating the inflammatory state of dental pulp tissue.

关 键 词：透明质酸 透明质酸合成酶 牙髓 炎症 

分 类 号：R781.31[医药卫生—口腔医学]