桃TCP转录因子家族的鉴定与分析  

Identification and Characterization of TCP Transcription Factors in Prunus persica

作　　者：韩继红 刘金凤 刘慧敏[2] Han Jihong;Liu Jinfeng;Liu Huimin(Puyang City Appearance and Environmental Sanitation Management Office,Puyang,457000;Key Laboratory of Non-timber Forest Germplasm En-hancement&Utilization of National Forestry and Grassland Administration,Non-timber Forest Research and Development Center,Chinese Academy of Forestry,Paulownia Research&Development Center of China,National Forestry and Grassland Administration,Zhengzhou,450003)

机构地区：[1]濮阳市市容环境卫生管理处,濮阳457000 [2]中国林业科学研究院经济林研究开发中心,国家林业和草原局泡桐研究开发中心,经济林种质创新与利用国家林业和草原局重点实验室,郑州450003

出　　处：《分子植物育种》2020年第16期5261-5267,共7页Molecular Plant Breeding

基　　金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2017ZA004-7)资助。

摘　　要：TCP基因家族是含有bHLH基序的植物特有转录因子,广泛参与植物的多种生物过程。为了解桃(Prunus persica)中TCP家族的成员和特征,本研究基于其全基因组组装及注释数据,首次在桃基因组中对TCP基因进行了系统鉴定,并进行了染色体定位、蛋白特征、基因结构、进化关系、启动子区域顺式元件分析。结果显示:桃基因组中共有20个TCP家族成员,在7条染色体上均有分布,其推导蛋白的长度、分子量和等电点差异较大。大部分TCP基因不含有内含子,基因结构较为简单。桃TCP基因家族含有植物TCP家族的所有类别,每一类别均含有保守的且能明显区别于其他类别的特征。TCP基因家族的启动子区域含大量光应答、激素响应等顺式作用元件,与该基因家族功能的多样性一致。本研究初步探索了桃基因组中TCP基因家族的特征,为后期该基因家族在桃中的功能验证提供了理论支撑。TCP genes encode plant-specific transcription factors with a bHLH motif and is widely involved in multiple biological processes in plants.In order to understand the members and characteristics of TCP family in peach,we systematically screened TCP genes in peach based on its genome assembly and annotation and analyzed their chromosome location,protein characteristics,gene structure,evolutionary relationship,and cis-elements in the promoter region.In total,20 members of TCP%familywere identified in the peach genome,which was distributed on seven chromosomes.The length,molecular weight,and isoelectric point of the deduced proteins were very different.The gene structures of TCPs were relatively simple since most of the TCPs contained no introns.Phylogenetic analysis and multiple sequence alignment indicated that the peach TCP family contained all classes of the plant TCP groups,each of which contained features that were conservative and could be clearly distinguished from other classes.The promoter regions of the TCP genes contained a large number of cis-acting elements,such as light response and hormone response,which is consistent with the diversity of functions of this family.We explored the characteristics of the TCP family in the peach genome,which provides theoretical support for the functional verification of this family in peach.

关 键 词：桃(Prunus persica) TCP基因家族 系统进化分析 转录因子 

分 类 号：Q943.2[生物学—植物学] S662.1[农业科学—果树学]