少年精神分裂症患儿精神分裂症断裂基因1多态性与脑结构的关系  

Relationship between disrupted-in-schizophrinia-1 polymorphism and the abnormal brain structure in childhood and adolescence with schizophrenia

作　　者：郭敬华[1] 衣志爽 郭素芹[1] 李玉玲[1] 梁颖慧[1] 夏艳红[1] 邵荣荣[1] 李杰[3] Guo Jinghua;Yi Zhishuang;Guo Suqin;Li Yuling;Liang Yinghui;Xia Yanhong;Shao Rongrong;Li Jie(Department of Child and Adolescent Psychiatry,the Second Affiliated Hospital of Xinxiang Medical University,Xinxiang 453002,Henan Province,China;Department of Diagnostics,Sanquan College of Xinxiang Medical University,Xinxiang 453002,Henan Province,China;Department of Radiology,the Second Affiliated Hospital of Xinxiang Medical University,Xinxiang 453002,Henan Province,China)

机构地区：[1]新乡医学院第二附属医院儿童少年精神科,河南新乡453002 [2]新乡医学院三全学院诊断学教研室,河南新乡453003 [3]新乡医学院第二附属医院放射科,河南新乡453002

出　　处：《中华实用儿科临床杂志》2020年第13期1009-1013,共5页Chinese Journal of Applied Clinical Pediatrics

基　　金：河南省重点科技攻关项目(132102310446,182102310155)。

摘　　要：目的调查精神分裂症断裂基因1(DISC1)rs4658971位点多态性与儿童少年精神分裂症的关系,及首发少年精神分裂症患儿DISC1基因rs4658971位点多态性与脑结构的关系。方法1.选择2010年10月至2018年12月在新乡医学院第二附属医院住院的577例儿童少年精神分裂症患者及516例健康儿童少年,采用限制性片段长度多态性方法(PCR-RFLP)检测其DISC1基因rs4658971多态性,采用χ^2检验比较精神分裂症患者与健康儿童少年DISC1基因型及等位基因频率的差异。2.选择118例首发、病程≤12个月的儿童少年精神分裂症患者和101例健康儿童少年进行3.0T磁共振成像(MRI)检测,采用协方差(年龄作协变量)分析不同DISC1基因型患者的颅脑结构。结果1.儿童少年精神分裂症患者与健康儿童少年比较,DISC1基因的rs4658971位点基因型频率(TT 6.6%比5.2%,TC 37.4%比38.4%,CC 56.0%比56.4%)比较,差异均无统计学意义(χ^2=0.91,P=0.63);等位基因频率比较(C 74.70%比75.58%,T 25.30%比24.42%),差异均无统计学意义(χ^2=0.23,P=0.63)。2.与健康儿童少年比较,首发儿童少年精神分裂症患者的第三脑室横径扩大[(4.68±1.41)mm比(4.06±1.36)mm],差异有统计学意义(F=8.534,P=0.004);侧脑室前脚间距、尾状核头部宽度、海马厚度均缩小[(30.69±2.51)mm比(32.02±2.41)mm]、[(8.17±1.01)mm比(9.22±1.14)mm]、[(8.93±0.97)mm比(9.93±1.14)mm],差异均有统计学意义(F=15.435、40.201、44.650,均P<0.001)。用协方差分析(用年龄作协变量)显示,DISC1 CC基因型患者与CT+TT基因型患者之间尾状核头部宽度的差异有统计学意义[(8.73±0.85)mm比(7.39±0.61)mm,F=41.376,P<0.001]。结论首发少年精神分裂症患儿存在多个脑结构改变,患者DISC1基因rs4658971位点等位基因T携带者可能与尾状核头部缩小有关。Objective To investigate the relationships of rs4658971 polymorphism of disrupted-in-schizophrinia-1(DISC1)gene with schizophrenia in children and adolescents and with the brain structure of such patients.Methods(1)A total of 577 children and adolescents with schizophrenia were recruited from the Inpatient Department of the Second Affiliated Hospital of Xinxiang Medical University from October 2010 to December 2018,and 516 healthy children were selected as the control group.The rs4658971 polymorphism of DISC1 gene was detected by restriction fragment length polymorphism(PCR-RFLP).The differences of DISC1 genotype and allele frequencies between schizophrenia patients and healthy children were compared by Chi-square test.(2)One hundred and eighteen patients with the first-episode schizophrenia whose illness duration was no more than 12 months and 101 healthy children and adolescents were detected by 3.0T magnetic resonance imaging(MRI).The craniocerebral structures of patients with different DISC1 genotypes were analyzed by covariance with age as the covariant.Results(1)The rs4658971 genotype frequencies(TT 6.6%vs.5.2%,TC 37.4%vs.38.4%,CC 56.0%vs.56.4%,χ^2=0.91,P=0.63)and allele frequencies(C 74.70%vs.75.58%,T 25.30%vs.24.42%,χ^2=0.23,P=0.63)of DISC1 gene in children and adolescents with schizophrenia were not significantly different from those in the healthy children.(2)Compared with the control group,the transverse diameter of the third ventricle was significantly enlarged in children and adolescent with schizophrenia[(4.68±1.41)mm vs.(4.06±1.36)mm,F=8.534,P=0.004],while the distance between the anterior feet of the lateral ventricle,the width of the caudate nucleus head and the thickness of the hippocampus were significantly reduced[(30.69±2.51)mm vs.(32.02±2.41)mm,(8.17±1.01)mm vs.(9.22±1.14)mm,(8.93±0.97)mm vs.(9.93±1.14)mm],and the differences were statistically significant(F=15.435,40.201,44.650,all P<0.001).The covariance analysis(with age as the covariant)showed that there was significant differe

关 键 词：精神分裂症 儿童少年 磁共振成像 精神分裂症断裂基因1 

分 类 号：R749.3[医药卫生—神经病学与精神病学]