具有拮抗葡萄灰霉菌的生防酵母菌分离与筛选  

Separating and Screening of Yeast against Postharvest Grape Gray Mold

作　　者：张栋园 王晓妍 李宁 潘巨忠 Zhang Dongyuan;Wang Xiaoyan;Li Ning;Pang Juzhong(College of Horticulture and Landscape,Tianjin Agriculture University,Tianjin 300384,China;Ningbo Yinzhou District Safe Food Technology Co.,Ltd.,Ningbo 315100,China)

机构地区：[1]天津农学院园艺园林学院,天津300384 [2]宁波市鄞州赛弗兑食品科技有限公司,浙江宁波315100

出　　处：《山东化工》2020年第15期42-43,49,共3页Shandong Chemical Industry

基　　金：大学生创新创业训练计划项目(国家级)(项目编号:201810061039);农业农村部农产品贮藏保鲜重点实验室开放基金(项目编号:kf2019005)。

摘　　要：本次实验从不同食材中分离筛选出21株酵母菌,通过平板对峙筛选发现10株酵母菌对草莓灰霉菌抑制效果超过50.00%,通过对峙培养发现酵母菌株SL-1和L-4的对葡萄灰霉菌菌丝体的抑制效果最显著,分别为66.04%和63.63%;从不同酵母菌菌株产挥发性物质对葡萄灰霉菌生长的影响的实验中发现酵母菌株SL-1和PG-2对葡萄灰霉菌的抑制效果最佳,达到71.56%和68.80%,由此可以看出,酵母菌株酵母菌株SL-1和PG-2对葡萄采后灰霉菌具有良好的抑制效果,其中以酵母菌株SL-1效果最显著,其次为酵母菌株PG-2。因此,酵母菌株SL-1和PG-2有望被进一步开发为葡萄灰霉菌的理想生防酵母菌。In this experiment,21 yeast strains were isolated from a variety of ingredientsand through confrontation training.10 strains of these have inhibition effect of more than 50.00% againstgrapegrey moldthrough plate confrontation,The results showed that SL-1and L-4had significant inhibitory effect on grapegrey mold,and its inhibition rate reached 66.04% and 63.63%;Volatile substance production was determined from 10 strains of yeast screened,The stains of SL-1and PG-2 with significant inhibitory effect on grapegrey mold was screened,and the inhibition rates reached 71.56% and 68.80%.It can be seen from thisstudy that yeast strains SL-1 and L-4 have good inhibitory effect on grape postharvestgrey mold,among which yeast strain SL-1 has the most significant effect,followed by yeast strain L-4.So it can be concluded that yeast strains SL-1 and L-4 are expected to be further developed as the ideal biocontrol yeast of grape grey mold.

关 键 词：葡萄灰霉菌 酵母菌 分离筛选 抑菌效果 

分 类 号：S436.631[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]