真武汤对心肾阳虚型心力衰竭大鼠心肌细胞保护的自噬机制研究  

Autophagy mechanism of Zhenwu decoction in myocardial cell protection of rats with heart-kidney and yang deficiency

作　　者：黄剑[1] 马晓彤 张亚杰[1] 李丽静[1] 韩冬[1] 刘佳[1] HUANG Jian;MA Xiaotong;ZHANG Yajie;LI Lijing;HAN Dong;LIU Jia(Changchun University of Traditional Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学药学院,长春130117

出　　处：《中国比较医学杂志》2020年第8期49-56,共8页Chinese Journal of Comparative Medicine

基　　金：中国博士后基金(2018M631838);吉林省教育厅“十三五”科学技术项目(JJKH20190476KJ)。

摘　　要：目的研究真武汤对心肾阳虚大鼠心肌细胞保护的自噬机制。方法手术摘除甲状腺及腹腔注射阿霉素,建立大鼠心肾阳虚模型,灌胃给药10 d。检测并记录各组大鼠的自然情况,采用ELISA法检测血清三碘甲状原氨酸(T3)、甲状腺素(T4)、促甲状腺激素(TSH)及心肌组织BNP、LA和CaN含量。Western blot考察LC3、ATG5、P62蛋白表达。免疫组化法测定大鼠心肌组织LC3和P62表达。HE染色观察心肌组织病理变化。结果真武汤可以降低心肌组织BNP含量,升高心肌组织LA含量,降低Ca N含量,升高心肌组织LC3、ATG5、BECLIN1含量,降低P62含量。结论真武汤对心肾阳虚所致心力衰竭情况具有改善作用,并且能够改善心脏能量代谢,在心力衰竭初期可以通过调节自噬保护损伤的心肌细胞。Objective To study autophagy as the protective mechanism of Zhenwu decotion in cardiomyocytes of rats with heart-yang deficiency.Methods After removal of the thyroid and intraperitoneal injection of doxorubicin,to establish the rat heart and kidney yang deficiency model,rats were administered with corresponding drugs for 10 days.We measured serum triiodothyronine(T3),thyroxine(T4),thyroid-stimulating hormone(TSH),and myocardial tissue BNP,LA,and CaN by ELISAs.LC3,ATG5,and P62 and protein expression was detected by western-blot.Inspected LC3 and P62 expression in rat myocardial tissue was examined by immunohistochemistry.ThePathological changes of myocardial tissue were observed by HE staining.Results Zhenwu decotion reduced the BNP content in myocardial tissue of rats with heart-yang deficiency-induced heart failure,increased the LA content in myocardial tissue,decreased the CaN content,increased LC3,ATG5,and BECLIN1 contents,and decreased the P62 content in myocardial tissue of rats with heart failure.Conclusions Zhenwu decotion improves the heart failure condition and energy metabolism of the heart.In the early stage of heart failure,the damaged cardiomyocytes protected by regulating autophagy.

关 键 词：真武汤 自噬 心肌细胞 机制研究 

分 类 号：R-33[医药卫生]