小鼠植入后胚胎体外延时培养体系的应用进展  

Advances in the application of an in vitro culture system of mouse post-implantation embryos

作　　者：邵红莲 谭韬 SHAO Honglian;TAN Tao(Faculty of Metallurgical and Energy Engineering,Kunming University of Science and Technology,Kunming 650093;China.2.Yunnan Key Laboratory of Primate Biomedical Research,Institute of Primate Translational Medicine,Kunming University of Science and Technology,Kunming 650500)

机构地区：[1]昆明理工大学生命科学与技术学院,昆明650500 [2]昆明理工大学云南中科灵长类生物医学重点实验室,昆明650500

出　　处：《中国比较医学杂志》2020年第8期119-124,共6页Chinese Journal of Comparative Medicine

摘　　要：小鼠是研究哺乳动物中一种小型的模式生物,在研究非人灵长类以及人类胚胎之前,需要先在小鼠体内确定实验能否进行再转移到高等哺乳动物。现有体系的限制使小鼠胚胎只能在体外培养至E6.5,小鼠胚胎体外延时培养体系的建立对于理解胚胎植入后的关键事件以及分子机制有着非常重要的作用,更为人胚胎的发育研究提供有效平台。单细胞测序技术的发展为小鼠胚胎体外延时培养提供了新的可能,在这里我们介绍了小鼠植入后胚胎的普通体外延时培养体系、3D培养体系、单细胞测序技术在胚胎领域的应用,以及其未来可能的发展方向。Mice are a small mammal model for scientific research.Before studying non-human primates and human embryos,it must be determined whether the experiment can be carried out in mice and then employ higher mammals.The limitation of the existing system is that mouse embryos can only be cultured in vitro to E6.5.Establishment of a mouse embryo in vitro culture system is very important to understand the critical events and molecular mechanisms of embryo periimplantation.The development of single-cell RNA sequencing technology provides new possibilities for in vitro time-lapse culture of mouse embryos.Here,we introduce an in vitro time-lapse culture system and 3D culture system for mouse embryo implantation,the application of single-cell RNA sequencing technology in the field of embryos,and its possible future direction of development.

关 键 词：小鼠胚胎发育 体外延时培养体系 3D培养体系 单细胞转录组测序 

分 类 号：R-33[医药卫生]