柚皮总黄酮的提取优化及其对亚硝酸盐清除作用的影响  

Extraction Optimization of Total Flavonoids from Pomelo Peel and Its Effect on Nitrite Removal

作　　者：谢建华[1,2] 陈思杨 张桂云 郑俊峰[1,2] 林理专 庞杰[3] XIE Jian-hua;CHEN Si-yang;ZHANG Gui-yun;ZHENG Jun-feng;LIN Li-zhuan;PANG Jie(Department of Food and Biology Engineering,Zhangzhou Profession and Technology Institute,Zhangzhou 363000,China;The Applied Technical Engineering Center of Further Processing and Safety of Agricultural Products,Higher Education Institutions in Fujian Province,Zhangzhou 363000,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]漳州职业技术学院食品与生物工程系,福建漳州363000 [2]农产品深加工及安全福建省高校应用技术工程中心,福建漳州363000 [3]福建农林大学食品科学学院,福建福州350002

出　　处：《长春师范大学学报》2020年第8期82-88,共7页Journal of Changchun Normal University

基　　金：福建省科技计划项目“漳州市食品产业技术研究院建设”(2018N2002)。

摘　　要：以柚皮为试材,分别研究了超声波辅助提取法单因素(提取时间、乙醇浓度、料液比)及碱提酸沉法单因素(提取时间、料液比、提取温度)对柚皮中黄酮类化合物提取率的影响。通过正交实验优化提取工艺,同时对提取的总黄酮进行清除亚硝酸盐和抗氧化实验。结果表明,碱提酸沉法提取的总黄酮量高于超声波辅助提取法,影响其提取率的因素依次为提取时间、料液比、提取温度,研究所得最佳工艺条件:提取时间1.5 h,料液比1∶25 g/mL,提取温度60℃。碱提酸沉法的柚皮黄酮的亚硝酸盐清除作用和抗氧化作用优于超声波辅助提取法。The effects of single factor(extraction time,ethanol concentration,material-liquid ratio)of ultrasonic assisted extraction method and single factor(extraction time,material-liquid ratio,extraction temperature)of alkaline extraction and acid precipitation method of the extraction rate of flavonoids in pomelo peel were studied,the extraction process was optimized by orthogonal experiment by using the optimal conditions of total flavonoids extract to remove nitrite.Results showed that the alkali mention acid sinking method compared with ultrasonic assisted extraction rate was high,the high yield of alkali,acid sinking method of extracting total flavonoids pomelo peel extraction yield factors were as follows:extraction time、material-liquid ratio、extraction temperature,the optimum technology of extracting temperature was 60℃,extracting time was 1.5 h,solid-liquid ratio was 1∶25 g/mL.The nitrite scavenging and anti-oxidation of total flavonoids by the alkali mention acid sinking method were superior to ultrasonic assisted extraction.

关 键 词：柚皮 总黄酮 亚硝酸盐 提取 清除作用 

分 类 号：TS202.3[轻工技术与工程—食品科学]