用于血液病毒核酸检测的微流控PCR温度控制技术研究  

Study on the temperature control technology of microfluidic PCR for the blood nucleic acid test

作　　者：李志刚[1,2] 吴晓松[1,2] 汪磊 刘勇[1] 朱灵[1] Li Zhigang;Wu Xiaosong;Wang Lei;Liu Yong;Zhu Ling(Institute of Applied Technology,Hefei Academy of Material Science,Chinese Acaderny of Sciences Hefei 230031,China;University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]中国科学院合肥物质科学院研究院应用技术研究所,合肥230031 [2]中国科学技术大学,合肥230026

出　　处：《电子测量技术》2020年第13期152-156,共5页Electronic Measurement Technology

基　　金：安徽省科技重大专项(17030801007);安徽省重点研发计划(201904c03020001)项目资助。

摘　　要：针对血液中病毒核酸快速检测的需求,研制用于微流控PCR扩增的温度控制系统。为了满足PCR反应所需的快速升降温和控温精确的要求,采用PT1000温度传感器和ADS1220高精度模数转换器,选用STM32F429作为主控芯片,提出Bang-Bang算法结合PID算法的复合算法,经过实验测试升降温速率分别为7.15℃/S和4.96℃/S,温控精度≤±0.1℃,温控系统完全满足HBV病毒核酸检测PCR扩增的需求。In this paper, we developed a temperature control system for microfluidic PCR amplification to satisfy the needs of rapid and accurate detection of virus nucleic acid in blood. In order to satisfy the requirements of rapid rise and fall, temperature control and precision for PCR reaction, the PT1000 temperature sensor and ADS1220 high-precision A/D converter are used. And STM32 F429 is selected as the main control chip. Besides all this, a composite algorithm of Bang-Bang algorithm and PID algorithm is proposed. The experimental results show that the rate of temperature rise and fall is 7.15 ℃/S and-4.96 ℃/S respectively, the temperature control accuracy is ≤±0.1 ℃, and the temperature control system fully satisfy the needs of HBV nucleic acid test.

关 键 词：微流控PCR 血液核酸检测 温度控制 复合控制算法 

分 类 号：TP271[自动化与计算机技术—检测技术与自动化装置]