母乳中活性多肽测定及对坏死性小肠结肠炎的预防作用分析  

Detection of active polypeptide in breast milk and its preventive effect on necrotizing enterocolitis

作　　者：田园园 张慧慧[1] 朱颖华 TIAN Yuan Yuan;ZHANG Hui Hui;ZHU Ying Hua(Zhengzhou Children’s Hospital Affiliated to Zhengzhou University(Henan Children’s Hospital,Zhengzhou Children’s Hospital)Zhenghzou,Henan Province,450052,China)

机构地区：[1]郑州大学附属儿童医院(河南省儿童医院郑州儿童医院),郑州450052

出　　处：《中国妇幼卫生杂志》2020年第4期75-77,共3页Chinese Journal of Women and Children Health

摘　　要：目的探讨母乳中活性多肽测定及对坏死性小肠结肠炎的预防作用。方法以45例早产产妇为对象,患者就诊时间段为2017年3月-2019年5月期间,于产妇产后2~5 d采集产妇母乳5 ml/次,分别持续6天采集每位产妇的母乳,使用超滤截流法分离早产儿产妇乳汁中的活性多肽进行测定,分析使用串联质谱法,比较坏死性小肠结肠炎致病菌单肠杆菌株-大肠杆菌标准菌株悬液,使用头孢哌酮舒巴坦溶液进行纸片法药敏试验,对母乳乳汁中的活性肽抑制病菌的作用进行实验。结果母乳乳汁经过测定发现4211条多肽,其中活性多肽有202条,非差异多肽有1440条。结论母乳中的复合活性多肽能有效抑制小肠结肠炎病菌,是预防坏死性小肠结肠炎的重要控制靶向指标,可以作为临床预防和治疗坏死性小肠结肠炎的重要依据。Objective To investigate the determination of active polypeptide in breast milk and its preventive effect on necrotizing enterocolitis.Methods Forty-five pregnant women with preterm delivery were selected from March 2017 to May 2019.After delivery 2-5 days,maternal breast milk 5ml/time was collected for 6 days.The active polypeptides in breast milk were separated by ultrafiltration abortion.The single pathogenic bacteria of necrotizing enterocolitis were analyzed by tandem mass spectrometry.The susceptibility test of standard strain suspension of Enterobacter Julius-Escherichia coli was carried out with cefoperazone sulbactam solution by disk method,and the effect of active peptide in breast milk on inhibiting pathogens was studied.Results 4211 polypeptides were detected in breast milk,including 202 active polypeptides and 1440 non-differential polypeptides.Conclusion The compound active polypeptide in breast milk can effectively inhibit the pathogen of enterocolitis,and is an important control target for the prevention of necrotizing enterocolitis.It can be used as an important basis for clinical prevention and treatment of necrotizing enterocolitis.

关 键 词：母乳活性多肽 坏死性小肠结肠炎 测定价值 抑菌活性 串联质谱 

分 类 号：R722.1[医药卫生—儿科]