GPC-3、HIF-1α和β-catenin在人肝细胞癌组织中的表达及临床意义  

Expression and correlation analysis of glypican-3,HIF-1αandβ-catenin in hepatocellular carcinoma

作　　者：桑伟[1] 楚慧[1] 古丽那尔·阿布拉江[1] 张巍[1] SANG Wei;CHU Hui;Gulina Abulajiang;ZHANG Wei(Department of Pathology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China)

机构地区：[1]新疆医科大学第一附属医院病理科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2020年第7期885-889,共5页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金资助项目(2017D01C312)。

摘　　要：目的研究磷脂酰肌醇蛋白多糖-3(GPC-3)、低氧诱导因子-1(HIF-1α)和β-连环蛋白(β-catenin)蛋白在人肝细胞癌(HCC)组织中的表达及临床意义。方法免疫组织化学方法检测GPC-3、HIF-1α和β-catenin 3种蛋白在96例HCC和28例正常肝组织石蜡标本的表达,分析3种蛋白表达的相关性,并结合HCC患者随访资料行临床病理参数与预后关系分析。结果GPC-3、HIF-1α和β-catenin在HCC中的表达分别为85.4%(82/96)、86.5%(83/96)和83.3%(80/96),在正常肝组织表达分别为0%(0/28)、17.9%(5/28)和3.6%(1/28),3种蛋白在HCC和正常肝组织表达差异均具有统计学意义(P<0.05),Spearman相关检验显示3种蛋白之间表达均有显著正相关性(P=0.000)。单因素分析显示GPC-3表达、肿瘤结节数目和癌栓与患者预后较差相关,Cox多因素回归分析显示肿瘤结节数目和癌栓是影响HCC术后独立预后因素。结论GPC-3、HIF-1α和β-catenin 3种蛋白在HCC中异常表达,并呈正相关。GPC-3可作为HCC预后评估的参考指标,肿瘤结节数目和癌栓是影响HCC患者预后的独立影响因素。Objective To study the expression and clinical significance of phosphatidylinositol proteoglycan-3(GPC-3),hypoxic inducible factor-1(HIF-1α)andβ-catenin proteins in human hepatocellular carcinoma(HCC)tissues.Methods The expression of three proteinsof GPC-3,HIF-1αandβ-catenin in paraffin specimens of 96 cases of HCC and 28 cases of normal liver tissue was detected by immunohistochemical method.The correlation between the expression of the three proteins was analyzed.The relationship between clinicopathological parameters and prognosis was analyzed according to the follow-up data of HCC patients.Results The expression of GPC-3,HIF-1αandβ-catenin in HCC was 85.4%(82/96),86.5%(83/96)and 83.3%(80/96),respectively,and that in normal liver tissue was 0%(0/28),17.9%(5/28)and 3.6%(1/28),respectively.There was significant difference between the three proteins in HCC and normal liver tissue(P<0.05).Spearman correlation test showed that there was a significant positive correlation between the expression of the three proteins(P=0.000).Univariate analysis showed that the expression of GPC-3,the number of tumor nodules and tumor thrombus were correlated with the poor prognosis.And Cox multivariate regression analysis showed that the number of tumor nodules and tumor thrombus were independent prognostic factors of HCC.Conclusion The abnormal proteinexpression of GPC-3,HIF-1αandβ-catenin in HCC tissues is positively correlated.GPC-3 can be used as a reference index for prognosis assessment of HCC,and the number of tumor nodules and tumor thrombus are independent prognostic factors of HCC.

关 键 词：肝细胞癌 GPC-3 HIF-1Α Β-CATENIN 

分 类 号：R735.7[医药卫生—肿瘤]