抗坏血酸对甘蔗原生质体生理生化及抗氧化和再生相关基因表达的调控  

Regulation of ascorbic acid on the physiology and biochemistry and the expression of genes related to oxidation and regeneration of sugarcane protoplasts

作　　者：李志刚[1] 赖沛衡 李素丽[1] 刘芳君 王令强 王爱勤[1] 龙安四[1] 韩世健[1] LI Zhigang;LAI Peiheng;LI Suli;LIU Fangjun;WANG Lingqiang;WANG Aiqin;LONG Ansi;HAN Shijian(College of Agriculture,Guangxi University,Nanning 530005,China)

机构地区：[1]广西大学农学院,南宁530005

出　　处：《植物生理学报》2020年第6期1248-1258,共11页Plant Physiology Journal

基　　金：国家自然科学基金(31871689和31460373);广西创新驱动重大专项(AA17204037-4)。

摘　　要：为减少甘蔗(Saccharum officinarum)体细胞培养和融合过程中原生质体的氧化褐变,本研究以‘新台糖22号’(ROC22)为材料,研究在KM8P液体培养基中添加抗坏血酸(L-ascorbic acid,ASA)对甘蔗原生质体活力的影响和机理。结果表明,添加不同浓度的ASA(0.5、1.5 mmol·L^-1)均可提高原生质体活力,降低丙二醛(MDA)含量,促进细胞壁再生,同时提高抗氧化相关酶基因(Cu/Zn SOD、CAT)和细胞壁基因(GAUT)、细胞周期基因(Cyclin A、Cyclin B和PSK)的表达水平。其中,添加0.5 mmol·L^-1的ASA时可获得最高活力的原生质体,更有利于细胞壁再生和抑制膜脂过氧化,激活抗氧化系统,同时促进细胞周期运转。In order to reduce the oxidative browning in sugarcane protoplast fusion process,sugarcane ROC22 protoplast was used as the object of study.Ascorbic acid(ASA)was added to KM8 P liquid medium to investigate the effects of ASA at different concentrations(0,0.5 and 1.5 mmol·L^-1)on cell viability and mechanism of sugarcane protoplasts.The results showed that the increase of protoplast activity,the decrease of MDA content,the promotion of cell wall regeneration,the increase of antioxidant related enzyme gene(Cu/Zn SOD and CAT),cell wall gene(GAUT)and cell cycle gene(cyclin A,cyclin B and PSK)can be achieved by adding ASA.Among them,0.5 mmol mmol·L^-1 ASA can obtain the most active protoplast,which was more conducive to cell wall regeneration,inhibition of membrane lipid peroxidation,activation of antioxidant system,and promotion of cell cycle operation.

关 键 词：甘蔗原生质体 抗坏血酸 活性氧 抗氧化酶 再生基因 

分 类 号：S566.1[农业科学—作物学]