MiR-146a在急性髓系白血病患者中的表达及意义  

Expression Level of MiR-146a in Acute Myeloid Leukemia Patients and Its Clinical Significance

作　　者：王毅力[1] 邓秀芝[1] 汤春静[1] 王晓毅[1] 于洁[1] WANG Yi-Li;DENG Xiu-Zhi;TANG Chun-Jing;WANG Xiao-Yi;YU Jie(Department of Hematology,Weihai Municipal Hospital,Weihai 264200,Shandong Province,China)

机构地区：[1]威海市立医院血液科,山东威海264200

出　　处：《中国实验血液学杂志》2020年第4期1171-1176,共6页Journal of Experimental Hematology

摘　　要：目的:检测微小RNA-146a(miRNA-146a)在急性髓系白血病(acute myeloid leukemia,AML)患者中的表达水平,评估其与AML患者临床特征、治疗应答、无事件生存期(event free survival,EFS)及总体生存期(overall survival,OS)的关联。方法:选取154例初发的AML患者纳入AML组,50例血小板减少性紫癜患者或骨髓捐赠志愿者纳入对照组。采用实时荧光定量聚合酶链式反应检测2组患者骨髓单个核细胞miR-146a的表达水平。同时评估AML患者接受化疗后的治疗应答、EFS和OS。结果:MiR-146a在AML组中表达水平明显低于对照组(P<0.01),受试者工作特征曲线(ROC)表明miR-146a可以很好地区分AML组和对照组受试者(曲线下面积:0.819,95%CI:0.761-0.877)。同时,miR-146a高表达患者的预后良好或中等的比例(P<0.01)、WBC≤15.2×10^9/L的比例(P<0.05),取得CR的比例(P<0.05),EFS(P<0.01)和OS(P<0.01)均显著高于miR-146a低表达患者。此外,Cox′s风险回归模型分析表明,miR-146a表达水平和EFS(P<0.01,HR=0.519)和OS(P<0.01,HR=0.478)呈正相关。结论:MiR-146a在AML患者骨髓单核细胞中表达水平显著低于对照者。骨髓单核细胞miR-146a可能是AML诊断和患者预后评估的重要生物学指标。Objective:To detect the expression of miR-146a in patients with AML,and to evaluate the relationship between miR-146a expression level and clinical characteristics,treatment response,EFS and OS.Methods:154 patients with newly diagnosed AML were enrolled in AML group,50 controls(patients with thrombocytopenic purpura or voluntary donor of bone marrow)were enrolled in control group.The miR-146a expression levels in bone marrow mononuclear cells was detected by RT-PCR between 2 group.AML patients were treated with chemotherapeutic drugs,and their clinical response and survivals were assessed.Results:The expression level of MiR-146a in AML group was significantly lower than that in control group.The ROC showed that miR-146a could distinguish the patients in AML and control group better(area under curve 0.819(95%CI:0.761-0.877).Meanwhile,the proportion of good and moderate good prognosis(P<0.001),proportion of WBC count≤15.2×10^9/L(P<0.05),CR rate(P<0.05),EFS(P<0.01)and OS(P<0.01)in patients with high miR-146a expression were higher than those in patients with low miR-146a expression.Cox′s model showed that miR-146a expression level positively realated with incressed EFS and OS.Conclusion:Mi R-146a is downregulated in AML patients,which might be served as a biomarker for predicting risk and prognosis of AML patients.

关 键 词：急性髓系白血病 MIR-146A 表达水平 治疗应答 预后 

分 类 号：R733.71[医药卫生—肿瘤]