下调Met表达对骨髓瘤RPMI 8226细胞生物学行为的影响  

Effect of Met Knockdown on Biological Behavior of Multiple Myeloma RPMI 8226 Cells

作　　者：刘慧丽[1] 上官㛃 阙文忠 LIU Hui-Li;SHANGGUAN Jie;QUE Wen-Zhong(Department of Medical Technology,ZhangZhou Health Vocational College,/Collaborative Innovation Center for Translation Medical Testing and Application Technology,Zhangzhou 363000,Fujian Province,China;Department of Anesthesiology,The Affiliated Nanping First Hospital of Fujian Medical University,Nanping 353000,Fujian Province,China;Department of Rheumatology,The Affiliated Nanping First Hospital of Fujian Medical University,Nanping 353000,Fujian Province,China)

机构地区：[1]漳州卫生职业学院医学技术系/转化医学检测应用技术协同创新中心,福建漳州363000 [2]福建医科大学附属南平第一医院麻醉科,福建南平353000 [3]福建医科大学附属南平第一医院风湿免疫科,福建南平353000

出　　处：《中国实验血液学杂志》2020年第4期1278-1282,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金青年科学基金项目(81500167);福建省自然科学基金面上项目(2019J01607);福建省科技创新联合资金项目(2018Y9123);福建省中青年教师教育科研项目(JZ180847);漳州卫生职业学院院本课题(ZWYZ201903);漳州卫生职业学院首届青年人才培育计划(QNRC201705)。

摘　　要：目的:探讨下调c-Met表达对多发性骨髓瘤RPMI 8226细胞增殖、凋亡和侵袭的影响。方法:将RPMI 8226细胞根据转染,分为对照组(RPMI8226细胞不做转染)、RPMI 8226/shRNA-control组和RPMI 8226/shRNA-Met组。采用Western blot检测c-Met蛋白表达水平以验证转染情况;采用MTT法检测细胞增殖;采用流式细胞术检测细胞周期和凋亡;与ECM(FN和Matrigel)和ECV304细胞的粘附检测其粘附能力;Transwell法检测细胞侵袭能力。结果:shRNA-Met可成功转染于RPMI 8226细胞,并有效抑制c-Met的表达,下调c-Met表达能有效抑制RPMI8226细胞增殖。与对照组相比较,shRNA-Met组在G_(0)/G_(1)期细胞比例和凋亡率(sub-G_(1))明显增加,而粘附率和迁移细胞的数量均明显下降;而与对照组相比,shRNA-control组各项指标均未见统计学差异(P>0.05)。结论:下调Met表达可影响骨髓瘤RPMI 8226细胞的增殖、粘附、侵袭和凋亡等生物学行为。Objective:To investigate the effects of down-regulating of c-Met expression to the proliferation,invasiveness and apoptosis of human multiple myeloma RPMI 8226 cells.Methods:According to transfection the RPMI8226 cells were dividide into RPMI 8226(untreated RPMI 8226),RPMI 8226/shRNA-Met and RPMI8226/shRNA-control group,respectively.Protein expression level of c-Met was detected by Western blot so as to evaluate transfection condition;the proliferation of the cells was detected by MTT;apoptosis and cycle of the cells were detected by flow cytometry;effect of c-Met/shRNA on RPMI 8226 cell adhesion was detected by RPMI 8226 cell adherence to ECM(Fn and Matrigel)and ECV304 cells.Invasiveness of RPMI 8226 cell was detected by Transwell assay.Results:The c-Met short hairpin RNA(shRNA)was successfully transfected into RPMI 8226 cells,and could inhibit the expression of c-Met significantly.The down-regulation of c-Met could inhibit the proliferation of RPMI 8226 cells significantly.The percentage of cells in the G_(0)/G_(1) phase and apoptotic rate(sub-G_(1))in the RPMI 8226/shRNA-Met group were higher than those in the control group,the adhesion rate and the number of migrated RPMI 8226/shRNA-Met cells were decreased significantly as compared with control group.There were no significant differences in each indexes between RPMI 8226/shRNA-control and control group.Conclusion:Knockdown of c-Met can affect the proliferation,adherence,invasiveness and apoptosis of human multiple myeloma RPMI 8226 cells.

关 键 词：多发性骨髓瘤 RPMI 8226细胞 生物学行为 C-MET 

分 类 号：R733.3[医药卫生—肿瘤] R329.2[医药卫生—临床医学]