硅胶柱层析法分离生鲜湿面酶促褐变产物  

Separation of Enzymatic Browning Products from Fresh Wet Noodles by Silica Gel Column Chromatography

作　　者：张亚茹 王远辉[1] 郭昱颖 许飞 李博宇 常永锋 张国治[1] ZHANG Yaru;WANG Yuanhui;GUO Yuying;XU Fei;LI Boyu;CHANG Yongfeng;ZHANG Guozhi(School of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001

出　　处：《食品科技》2020年第6期191-197,共7页Food Science and Technology

基　　金：河南工业大学科学研究基金自然科学项目(青年支持计划)(2017QNJH16);河南工业大学“科教融合”项目(26400030);河南省重点研发与推广专项科技攻关项目(182102110342)。

摘　　要：探究硅胶柱层析法中硅胶粒度、径高比、上样量和流速等因素对生鲜湿面酶促褐变产物分离效果的影响。结果表明:硅胶柱分离时采用分级洗脱的方式,最佳参数为硅胶粒度200~300目、径高比1:16、上样质量比为样品:硅胶=1:1000(g:g)、流速0.5 BV/h,洗脱体系为丙酮:水=1:0、20:1、10:1、5:1、2:1、1:1。分离得到6个组分,HPLC初步分析发现其中2个组分的纯度较高,分别为92.33%和93.39%。利用硅胶层析柱可将生鲜湿面酶促褐变产物进行分离,获得纯度较高的化合物,为其单体分离和分子结构解析提供参考。The effect of silica gel column chromatography used in the separation of enzymatic browning products from fresh wet noodles was investigated,and test parameters were the particle size of silica gel,the ratio of diameter to height,loading amount and flow rate.The results showed that fractional elution was used in the separation procedure of silica gel column,and the optimal parameters were as follows:the particle size of silica gel was 200~300 mesh,the diameter-to-height ratio was 1:16,the ratio of sample to silica gel was 1:1000(g:g),flow rate was 0.5 BV/h,the elution system was the solution of acetone and water(1:0,20:1,10:1,5:1,2:1,1:1).Six groups were isolated,and HPLC preliminary analysis showed that two of them had high purity,which were 92.33%and 93.39%,respectively.The enzymatic browning products of fresh wet noodles can be separated by silica gel chromatography column,and the compounds with high purity can be obtained,which can provide reference for monomer separation and molecular structure analysis.

关 键 词：分级洗脱 分离 优化 高效液相色谱 

分 类 号：TS213.24[轻工技术与工程—粮食、油脂及植物蛋白工程]