亚低温对重型颅脑损伤大鼠脑组织TRPM2、caspase9表达的影响  

Effect of Mild Hypothermia on the Expression of TRPM2 and Caspase-9 in the Brain Tissue of Rats with Severetraumatic Brain Injury

作　　者：刘洪军[1] 漆建[1] 尹久 苟章洋[1] 陆星宇 Liu Hongjun;Qi Jian;Yin Jiu(Department of Neurosurgery,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,China)

机构地区：[1]川北医学院附属医院神经外科,四川南充637000

出　　处：《四川医学》2020年第7期680-684,共5页Sichuan Medical Journal

摘　　要：目的采用改良的Feeney s自由落体损伤装置建立重型颅脑损伤模型,运用此模型探讨亚低温对重型颅脑损伤后大鼠TRPM2、Caspase-9表达影响。方法SPF(specific pathogen free)级成年SD(sprague-dawley)雄性大鼠108只,体质量280~340 g。随机分为3组,对照组(即仅钻孔)36只、颅脑损伤组(TBI组即仅钻孔打击)36只,颅脑损伤和亚低温组(TBI+MHT组)36只,各组根据打击后不同时间点,分为6个亚组,分别为打击后6 h、12 h、24 h、48 h、72 h和168 h,每个时间点6只大鼠。损伤后不同时间点分别进行改良大鼠神经功能缺损评分(mNSS),检测TRPM2、Casepase-9的表达。结果①大鼠mNSS评分结果:TBI组及TBI+MHT组在伤后呈先升高后降低的趋势,在24 h时间点达到高峰。②免疫组化法结果:TRPM2、Casepase-9的阳性细胞表达随时间的变化而变化,在伤后6 h、12 h、24 h、48 h、72 h、168 h的各时间点,阳性细胞的表达呈现出先升高后降低的趋势,伤后6 h即有升高,24 h达到高峰,伤后168 h降至最低。TBI+MHT组与TBI组比较各时间点差异有统计学意义(P<0.05)。结论颅脑损伤后的氧化应激可使TRPM2、Caspase-9表达增加,促使细胞凋亡,加重继发性脑损伤,亚低温治疗均对重型颅脑损伤具有脑保护作用。Objective To establish severe craniocerebral injury model with a modified Feeney s free-fall injury device,and to explore the effect of mild hypothermia on the expression of TRPM2 and Caspase-9 in rats after severe traumatic brain injury.Methods 108 specific pathogen free(SPF)Sprague-Dawley(SD)male rats with 280~340 g were randomly divided into 3 groups:control group(drilling only,n=36),traumatic brain injury group(TBI group,drilling and blowing only,n=36),traumatic brain injury and mild hypothermia group(TBI+MHT group,n=36).Each group was divided into 6 subgroups according to different time points,namely at 6 h,12 h,24 h,48 h,72 h and 168 h after the shock,and 6 rats were at each time point.At different time points,the modified rat neurological deficit score(mNSS)was measured and the immunohistochemical method was used to detect the expression of TRPM2 and Casepase-9.Results①Rat mNSS score results:TBI group and TBI+MHT group showed a trend of increasing first and then decreasing after injury,and a peak reached at 24 h.②Immunohistochemical results:the expression of TRPM2 and Casepase-9 positive cells changed with time.At 6 h,12 h,24 h,48 h,72 h and 168 h after injury,the expression of positive cells showed a trend of first increasing and then decreasing that it increased at 6 h after injury,reached a peak at 24 h,and decreased to a minimum at 168 h after injury.Compared with TBI group,TBI+MHT group had significant difference at each time point(P<0.05).Conclusion Oxidative stress after TBI could increase the expression of TRPM2 and Caspase-9,promote cell apoptosis and aggravate secondary brain injury.However,mild hypothermia treatment has a brain protective effect on severe TBI.

关 键 词：重型颅脑损伤 亚低温 TRPM2 CASPASE-9 细胞凋亡 大鼠 

分 类 号：R-332[医药卫生]