基质金属蛋白酶基因多态性与涎腺腺样囊性癌患者预后的相关性研究  

Study on the correlation between the single nucleotide polymorphism of matrix metalloproteinase gene and prognosis of patients with salivary adenoid cystic carcinoma

作　　者：王锦锋 李晓琰 任鹏飞 张晨[1] 李斌[1] 张媛[1] 秦红霞[1] WANG Jinfeng;LI Xiaoyan;REN Pengfei;ZHANG Chen;LI Bin;ZHANG Yuan;QIN Hongxia(Department of Preventive Dentistry,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,He’nan,China;Department of Dentistry,Children’s Hospital Affiliated of Zhengzhou University(Zhengzhou Children’s Hospital),Zhengzhou 450018,He’nan,China;Department of Molecular Pathology,the Affiliated Cancer Hospital of Zhengzhou University,Zhengzhou 450008,He’nan,China)

机构地区：[1]郑州大学第一附属医院口腔预防科,郑州450000 [2]郑州大学附属儿童医院(郑州儿童医院)口腔科,郑州450018 [3]郑州大学附属肿瘤医院分子病理科,郑州4500080

出　　处：《癌症进展》2020年第15期1547-1550,共4页Oncology Progress

摘　　要：目的探讨基质金属蛋白酶(MMP)基因MMP2和MMP9单核苷酸多态性(SNP)与涎腺腺样囊性癌(SACC)患者预后的关系。方法收集92例SACC患者外周血DNA,通过生物信息学筛选MMP2和基因功能性SNP并进行基因分型检测。结合临床信息和随访资料,利用Kaplan-Meier模型分析患者生存情况,Logrank检验比较生存曲线。结果临床分期为Ⅲ~Ⅳ期、有周围神经侵犯的SACC患者死亡率分别明显高于临床分期为Ⅰ~Ⅱ期、无周围神经侵犯的SACC患者,差异均有统计学意义(P<0.01)。MMP2基因rs243832野生型(GG)患者死亡率高于突变型(GC+CC)患者,差异有统计学意义(P<0.05)。rs243832位点GG基因型患者中位生存时间为69.1个月,短于GC+CC基因型患者的94.8个月,差异有统计学意义(P<0.05)。结论临床中尚缺少SACC患者预后诊断标志物,MMP基因SNP有望成为一种新型标志物用于SACC预后评估,指导临床术后治疗。Objective To investigate the relationship between single nucleotide polymorphisms(SNP) of matrix metalloproteinases(MMP)2 and MMP9 and overall survival of patients with salivary adenoid cystic carcinoma(SACC).Method The peripheral blood DNA of 92 SACC patients was collected,and the functional SNP of MMP2 and MMP9 genes were screened by bioinformatic assay and their genotyping was performed.The Kaplan-Meier model and Log-rank test were respectively used to assess the overall survival and related survival curve on the basis of clinical and follow-up data.Result The mortality rate of SACC patients with clinical stage Ⅲ-Ⅳ and peripheral nerve invasion was significantly higher than that of SACC patients with clinical stage Ⅰ-Ⅱ and no peripheral nerve invasion,the difference was statistically significant(P<0.01).The mortality rate of patients with gene rs243832 wild-type(GG) was higher than that of mutant type(GC+CC),and the difference was statistically significant(P<0.05).The median survival of patients with GG genotype at rs243832 loci was 69.1 months,which was shorter than the 94.8 months of patients with GC+CC genotype,and the difference was statistically significant(P<0.05).Conclusion There exists a lack of diagnostic markers for the prognosis of SACC patients in the clinical practice,and SNP in the MMP gene is expected to become a new kind of marker for the prognosis prediction of SACC and provide guidance for clinical postoperative treatment.

关 键 词：涎腺 腺样囊性癌 单核苷酸多态性 基质金属蛋白酶 预后 

分 类 号：R739.87[医药卫生—肿瘤]