HPLC法测定不同贮藏条件下莪术中6种化学成分的含量  

Content Determination of Five Chemical Components in Curcuma Rhizoma under Different Storage Conditions by HPLC

作　　者：周秋明 王文华 夏平 杨洪 彭薪颖 蒋德军 Zhou Qiumin;Wang Wenhua;Xia Ping;Yang Hong;Peng Xinying;Jiang Dejun(Traditional Chinese Medicine Department,Chongqing Dazu District People’s Hospital,Chongqing 402360,China;Pharmacy Department,Chongqing Dazu District People’s Hospital,Chongqing 402360,China)

机构地区：[1]重庆市大足区人民医院中医科,重庆402360 [2]重庆市大足区人民医院药学部,重庆402360

出　　处：《中国药师》2020年第8期1669-1673,共5页China Pharmacist

摘　　要：目的:建立同时测定不同贮藏条件下莪术中吉马酮、莪术二酮、莪术醇、β-榄香烯、姜黄素、去甲氧基姜黄素含量的方法。方法:采用HPLC法,色谱柱为WATERS Atlantisd C18(250 mm×4.6 mm,5μm),以0.3%甲酸乙腈溶液(A)-水溶液(B)为流动相,梯度洗脱;检测波长:215 nm(检测吉马酮、莪术二酮、莪术醇、β-榄香烯),420 nm(检测姜黄素和去甲氧基姜黄素);流速:1.0 ml·min^-1,柱温:25℃,进样量:10μl。结果:吉马酮、莪术二酮、莪术醇、β-榄香烯、姜黄素、去甲氧基姜黄素分别在的线性范围分别为0.080 3~0.884 2 mg·ml^-1、0.037 7~0.415 5 mg·ml^-1、0.018 3~0.201 1 mg·ml^-1、0.047 5~0.523 4mg·ml^-1、0.005 2~0.057 2 mg·ml^-1、0.003 1~0.034 3 mg·ml^-1范围内有良好线性(r=0.999 3~0.999 8)。精密度、稳定性、重复性试验的RSD均小于5%(n=6),平均加样回收率在98.09%~101.48%范围内(RSD <1.2%,n=6)。6批莪术药材中吉马酮、莪术二酮、莪术醇、β-榄香烯、姜黄素、去甲氧基姜黄素的含量分别为7.893 6~8.956 2 mg·ml^-1、7.893 6~8.956 2 mg·ml^-1、0.981 2~1.119 9 mg·ml^-1、3.000 5~3.199 7 mg·ml^-1、0.091 9~0.098 5 mg·ml^-1、0.064 7~0.073 5 mg·g^-1,均呈下降趋势,且下降速率为室温> 15℃> 5℃;在相同贮藏温度下的下降速率为聚乙烯塑料袋<聚丙烯编织袋;莪术醇及β-榄香烯的含量分别为0.981 2~1.119 9 mg·ml^-1、3.000 5~3.199 7 mg·ml^-1,反而有所增加。结论:该法简便、准确,用于同时测定莪术中6种成分的含量。建议莪术药材密封包装后于干燥阴凉处贮藏,且贮藏时间不宜过长。Objective: To establish the method for the content determination of germacrone,curdione,curcumol,-elemene,curcumin and demethoxycurcumin in Curcuma Rhizoma under different storage conditions. Methods: An HPLC method was adopted. The determination was performed on a WATERS Atlantisd C18( 250 mm × 4.6 mm,5 μm) column with the mobile phase consisting of 0.3% acetonitrile formate solution( A)-water( B)( gradient elution) at the flow rate of 1.0 ml·min^-1. The detection wavelength was set at 215 nm,and the column temperature was 25 ℃ . The sample size was 10 μl. Results: The linear range of germacrone,curdione,curcumol,-elemene,curcumin and demethoxycurcumin was 0.080 3-0.884 2 mg·ml^-1,0.037 7-0.415 5 mg·ml^-1,0.018 3-0.201 1 mg·ml^-1,0.047 5-0.523 4 mg·ml^-1,0.005 2-0.057 2 mg·ml^-1 and 0.003 1-0.034 3 mg·ml^-1( r =0.999 3-0.999 8),respectively. The RSDs of precision,stability and repeatability tests were all less than 5%( n = 6). The recoveries were 98.09%^-101.48%( RSD < 1.2%,n = 6). The content range of 6 batches of samples were 7.893 6-8.956 2 mg·g^-1,7.893 6-8.956 2 mg·g^-1,0.981 2^-1.119 9 mg·g^-1,3.000 5-3.199 7 mg·g^-1,0.091 9-0.098 5 mg·g^-1 and 0.064 7-0.073 5 mg·g^-1,respectively,showing a decreasing trend;the decrease rate in descending order was room temperature > 15℃ > 5℃ . At the same storage temperature,the decreasing rate of Curcumol and β-elemene were 0.981 2^-1.119 9 mg·ml^-1 and 3.000 5-3.199 7 mg·ml^-1 respectively,but they increased.Conclusion: The method is simple,accurate and feasible,and can be used for the simultaneous determination of 6 components in curcuma rhizoma. It is suggested that curcuma rhizoma should be sealed and packed in dry and cool places and should not be stored for a long time.

关 键 词：莪术 贮藏条件 高效液相色谱法 吉马酮 莪术二酮 莪术醇 Β-榄香烯 姜黄素 去甲氧基姜黄素 含量测定 

分 类 号：R927.2[医药卫生—药学]