针刀松解对坐骨神经痛大鼠中枢多巴胺、去甲肾上腺素及5-羟色胺的影响  

Effects of Acupotomy Release on Central DA, NE and 5-HT in Rats with Sciatica

作　　者：张立 董玮 董介轩 殷志荣 石小东 李皎 刘维统 ZHANG Li;DONG Wei;DONG Jiexuan;YIN Zhirong;SHI Xiaodong;LI Jiao;LIU Weitong(Department of Orthopedics and Traumatology,Kunming Hospital of Traditional Chinese Medicine,the Third Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming 650032,China)

机构地区：[1]云南中医药大学第三附属医院(昆明市中医医院)骨伤科,昆明650032

出　　处：《中国中医骨伤科杂志》2020年第8期13-16,共4页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：云南省医疗卫生单位内设研究机构科研项目(2016NS103);2017云南省科技计划项目(2017FF117-(-048))。

摘　　要：目的:观察针刀松解法治疗坐骨神经痛大鼠对其延髓、丘脑、海马、腰膨大脊髓多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)及热缩足阈值的影响。方法:将60只SD大鼠随机分为假手术组、神经结扎组及针刀治疗组。假手术组仅手术暴露坐骨神经,不做结扎;神经结扎组手术暴露并结扎坐骨神经;针刀治疗组手术结扎坐骨神经,在造模成功后第7,14,21天分别进行针刀松解治疗。3组在第28天进行热缩足试验,测定大鼠热缩足阈值;处死大鼠取延髓、丘脑、海马、腰膨大脊髓组织;用ELISA法检测DA,5-HT及NE浓度。结果:针刀治疗组与假手术组对比,大鼠腰膨大脊髓、丘脑5-HT,NE,DA浓度高于假手术组,差异有统计学意义(P<0.01);延髓5-HT,NE,DA浓度低于假手术组,差异有统计学意义(P<0.01)。针刀治疗组与神经结扎组对比,大鼠腰膨大脊髓5-HT,NE,DA浓度高于神经结扎组,延髓5-HT,NE,DA浓度低于神经结扎组,差异有统计学意义(P<0.01)。结论:针刀松解治疗坐骨神经痛大鼠能促进中枢DA,NE,5-HT在相应抑制疼痛受体分布区域的表达,起中枢镇痛的作用。Objective:To observe the effects of acupotomatolysis on dopamine(DA),norepinephrine(NE),5-hydroxytryptamine(5-HT)and thermal foot threshold in sciatica rats.Methods:60 SD rats were randomly divided into sham operation group,nerve ligation group and acupotomology group.In the sham operation group,the sciatic nerve was exposed surgically without ligation.Nerve ligation group:rats accepted surgical exposure and ligation of the sciatic nerve.Acupotomology group:surgical ligation of sciatic nerve,acupotomology release treatment 7,14 and 21 d after successful modeling.The thermal foot shrinkage test was conducted in the three groups on 28 d to determine the threshold of thermal foot shrinkage in the rats.The rats were sacrificed and the medulla oblongata,thalamus,hippocampus and lumbar dilated spinal cord were collected.DA,5-HT and NE concentrations were determined by ELISA.Results:The concentrations of spinal cord enlargement of thalamus 5-HT,NE and DA in the acupotomology group were higher than those in the sham group(P<0.01).The concentrations of 5-HT,NE and DA in medulla oblongata were lower than those in the sham group(P<0.01).The concentrations of 5-HT,NE and DA in the lumbar enlargement spinal cord of acupotomology groupwere higher than those in the nerve ligation group,and the concentrations of 5-HT,NE and DA in the medulla oblanceolata were lower than those in the nerve ligation group(P<0.01).Conclusion:Acupotomy release therapy for sciatica rats can promote the expression of DA,NE and 5-HT in the corresponding pain receptor distribution area,and play the role of central analgesia.

关 键 词：坐骨神经痛 针刀松解法 中枢镇痛 

分 类 号：R-33[医药卫生]