生骨胶囊对骨质疏松大鼠骨组织骨保护素及核因子KB受体活化因子配基表达的影响  

Effect of Raw Bone Capsule on the Expression of OPG and RANKL in Tibia of Osteoporotic Rats

作　　者：张旭桥 张信成[2] 仇湘中[2] 姜升平 黄超文 王晓晖 ZHANG Xuqiao;ZHANG Xincheng;QIU Xiangzhong;JIANG Shengping;HUANG Chaowen;WANG Xiaohui(Shenzhen Hospital Beijing University of Traditional Chinese Medicine(Longgang),Shenzhen 518172,Guangdong China;Affiliated Hospital of Hunan Institute of Traditional Chinese Medicine,Changsha 410006,China;Xiangtan Hospital of Traditional Chinese Medicine,Xiangtan 411100,Hunan China)

机构地区：[1]北京中医药大学深圳医院(龙岗),广东深圳518172 [2]湖南省中医药研究院附属医院 [3]湘潭市中医医院

出　　处：《中国中医骨伤科杂志》2020年第8期21-25,共5页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：湖南省中医药科研基金2014年资助项目(2014126);全国名老中医仇湘中教授传承工作室专项资金资助(国中医药人教发[2016]42号文);全国中医临床特色技术传承骨干人才专项资金资助(国中医药人教函[2019]36号文)。

摘　　要：目的:探讨生骨胶囊对骨质疏松大鼠胫骨组织中骨保护素(OPG)及核因子KB受体活化因子配基(RANK)表达的影响,分析其改善骨质疏松的作用机制。方法:建立36只骨质疏松大鼠模型,随机分为正常对照组、假手术组、模型组、生骨胶囊组、骨疏康颗粒组和尼尔雌醇片组,每组6只,生骨胶囊组予以生骨胶囊灌胃;其他组分别予等剂量生理盐水、骨疏康颗粒和尼尔雌醇片治疗。分别于给药后第3周和第6周每组随机抽取3只,处死大鼠取患处组织标本,采用染色后视野下的阳性表达部位的累积光密度和视野下样品面积的比值法测定大鼠骨组织中OPG和RANK的含量,对所得数据进行统计学分析。结果:生骨胶囊组大鼠在第3周及第6周末患处骨组织标本中OPG和RANK表达均优于其他组,差异有统计学意义(P<0.05)。结论:生骨胶囊可通过改善骨质疏松骨组织中OPG和RANK的表达,从而达到改善骨质疏松的作用。Objective:To investigate the effect of raw bone capsule on the expression of OPG and RANK in tibia of osteoporotic rats,and to analyze the mechanism of raw bone capsule in improving osteoporosis.Methods:36 osteoporosis rat models were established and randomly divided into normal control group,sham-operated group,model group,raw bone capsule group,Gushukang granule group and Nilestriol tablet groupwith 6 rats in each group.Raw bone capsule group was given raw bone capsule by gastric perfusion;The normal control group,sham-operated group and model group were given the same dose of physiological saline.The Gushukang granule group was given the same dose of Gushukang granules;The Nilestriol tablet group was given the same dose of Nilestriol tablet.At the 3 rd and 6 th week after administration,3 rats were randomly selected from each group detected.The contents of OPG and RANK in bone tissue of rats were determined by the ratio of cumulative light density of the positive expression site in the vision field after staining and the area of the sample in the vision field.The data were analyzed statistically.Results:The expressions of OPG and RANK in bone tissue samples of Raw bone capsule group were higher than those of other control groups at the 3 rd and 6 th week(P<0.05).Conclusion:Raw bone capsule can promote the expression of OPG and RANK in osteoporotic bone tissue and improve osteoporosis.

关 键 词：生骨胶囊 骨质疏松 骨保护素 核因子KB受体活化因子配基 大鼠 

分 类 号：R-33[医药卫生]