分泌型卷曲相关蛋白2抑制人结直肠癌细胞系SW480的增殖和迁移  

Secreted frizzled-related protein 2 inhibits the proliferation and migration of human colorectal cancer cell line SW480

作　　者：周菁 李索妮 ZHOU Jing;LI Suo-ni(Department of Oncology, Shaanxi Province Tumor Hospital, Xi'an 710061, China)

机构地区：[1]陕西省肿瘤医院肿瘤内科,陕西西安710061

出　　处：《基础医学与临床》2020年第9期1212-1217,共6页Basic and Clinical Medicine

基　　金：陕西省重点研发计划项目(2017SF-080)。

摘　　要：目的探究分泌型卷曲相关蛋白2(SFRP2)抑制人结直肠癌细胞系SW480增殖、迁移的分子机制。方法在人正常结肠上皮细胞HCoEpiC和结直肠癌细胞系SW480中采用Western blot和RT-qPCR检测SFRP2的表达;构建shSERP2稳转SW480细胞株;在shNC SW480和shSFRP2 SW480细胞中采用Western blot和RT-qPCR检测SFRP2、低氧诱导因子-1α(HIF-1α)和Twist的表达;HIF-1α抑制剂(IDF-11774)刺激shSFRP2 SW480细胞后,CCK-8法检测细胞增殖,Transwell小室法检测细胞迁移、侵袭。结果SW480细胞中SFRP2的表达量低于HCoEpic细胞(P<0.01);敲降SFRP2后,SW480细胞增殖、迁移、侵袭能力显著提高(P<0.001);而细胞内HIF-1α和Twist表达水平显著提高(P<0.01);HIF-1α抑制剂(IDF-11774)刺激后,其增殖、迁移、侵袭能力显著回降(P<0.001)。结论SFRP2通过调控HIF-1α-Twist信号轴发挥其抑癌作用。Objective To explore the molecular mechanism by which secreted frizzy-related protein 2(SFRP2)inhibits proliferation and migration of human colorectal cancer cell line SW480.Methods The expression of SFRP2 was detected by Western blot and RT-qPCR in human normal colonic epithelial cells HCoEpiC and colorectal cancer cell line SW480.The shSERP2 SW480 cell strain was constructed.Western blot and RT-qPCR were used to detect the expression of SFRP2,hypoxia inducible factor-1α(HIF-1α),and Twist in shNC SW480 and shSFRP2 SW480 cells.After shSFRP2 SW480 cells were stimulated by HIF-1αinhibitor(IDF-11774),CCK-8 assay detected cell proliferation and Transwell detected cell migration and invasion.Results The expression level of SFRP2 in SW480 cells was lower than that in HCoEpiC cells(P<0.01).After SFRP2 knockout,the proliferation,migration and invasion ability of SW480 cells were significantly improved(P<0.001).The expression of HIF-1αand Twist in SW480 cells was increased(P<0.01).After HIF-1αinhibitor(IDF-11774)treatment of shSFRP2 SW480 cells,compared with the untreated group,the proliferation,migration and invasion ability of shSFRP2 SW480cells were significantly decreased(P<0.001).Conclusions SFRP2 exerts certain anticancer effects through regulation of the HIF-1α/Twist signaling axis.

关 键 词：结直肠癌 分泌型卷曲相关蛋白2(SFRP2) 低氧诱导因子-1α(HIF-1α) TWIST 

分 类 号：R735.37[医药卫生—肿瘤]