不同穴位电针对阿尔茨海默症大鼠的疗效及其机制研究  

Effect of electroacupuncture of different acupoint groups on learning-memory ability and expression of IL-1βand TNF-αin hippocampus and prefrontal cortex in rats with Alzheimer’s disease

作　　者：王依滢 吴旋 唐成林[3] 许毅[4] 王健蓉[5] 徐进[1] 黄娟[1] 邱国平[1] 盛华均[1] 朱淑娟[1] WANG Yi-ying;WU Xuan;TANG Cheng-lin;XU Yi;WANG Jian-rong;XU Jin;HUANG Juan;QIU Guo-ping;SHENG Hua-jun;ZHU Shu-juan(Department of Human Anatomy,Chongqing Medical University,Chongqing,400016,China;Department of Neurosurgery,The Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010;College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing,400016;College of Medicine,Chengdu University,Chengdu 610106;Department of Neurology,The Fifth People’s Hospital of Chengdu,Chengdu 611130)

机构地区：[1]重庆医科大学人体解剖教研室,重庆400016 [2]重庆医科大学附属第二医院神经外科,重庆400010 [3]重庆医科大学中医药学院,重庆400016 [4]成都大学医学院,成都610106 [5]成都市第五人民医院神经内科,成都611130

出　　处：《针刺研究》2020年第8期617-622,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81601051、81273870);重庆市市科委项目(No.cstc2017jcyjAX0414、cstc2016jcyjA0229);重庆市教委项目(No.KJ1600213)。

摘　　要：目的:观察并比较"益肾调督"电针法和"补肺益肾调督"电针法对阿尔茨海默症(AD)大鼠学习和记忆能力的改善情况,以及对大鼠海马及前额叶皮层部分炎性因子的影响,对其相关机制进行初步探讨。方法:将SD大鼠随机分为对照组、假手术组、模型组、两穴位治疗组和三穴位治疗组,每组各8只。于双侧海马注射10μLβ-淀粉样蛋白(Aβ25-35)制作AD模型,假手术组注射等量的0.9%氯化钠溶液。两穴位治疗组造模成功后电针"百会"和"肾俞",三穴位治疗组造模成功后电针"百会""肾俞"和"肺俞",1次/d,共10 d。通过Morris水迷宫实验观察各组大鼠的学习和记忆能力变化,用Western blot法检测各组大鼠前额叶皮层和海马组织中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)的表达变化。结果:造模后大鼠饮食、行动等一般情况较差,干预后两治疗组一般情况均较模型组有所好转。水迷宫实验结果显示,与对照组和假手术组比较,模型组大鼠平均逃避潜伏期明显延长(P<0.05),穿越原平台的次数明显减少(P<0.05);与模型组比较,两治疗组平均逃避潜伏期均明显缩短(P<0.05),穿越原平台次数均明显增加(P<0.05)。Western blot结果显示,与对照组和假手术组比较,模型组大鼠前额叶皮层和海马组织中IL-1β、TNF-α蛋白的相对表达量明显升高(P<0.0001,P<0.001,P<0.01);与模型组比较,两治疗组IL-1β、TNF-α相对表达量均明显降低(P<0.01,P<0.05)。结论:无论是"益肾调督"电针法还是"补肺益肾调督"电针法均可改善AD模型大鼠的一般情况和学习记忆能力,且电针干预可抑制AD模型大鼠前额叶皮层和海马组织中IL-1β、TNF-α表达的增加,这可能是电针治疗AD的潜在机制之一。Objective To compare the effect of electroacupuncture(EA)of acupoint group for"reinforcing the kidney and regulating Governor Vessel"and acopoint group for"reinforcing the kidney and lung and regulating Governor Vessel"on lear-ning-memory ability and expression of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)proteins in the hippocampus and prefrontal cortex(PFC)in Alzheimer’s disease(AD)rats,so as to explore the efficacy of the two acupoint groups and mechanisms underlying improvement of AD.Methods Forty male SD rats were randomly divided into control,sham operation,model,"Baihui"+"Shenshu"(GV20+BL23,for"reinforcing the kidney and regulating Governor Vessel")EA and GV20+BL23+"Feishu"(BL13,GV20+BL23+BL13,for"reinforcing the kidney and lung and regulating Governor Vessel")EA groups(n=8 rats in each group).The AD model was established by bilateral injection of amyloidβpeptide(Aβ25-35,10μL)into bilateral hippocampus,and rats of the sham operation group received injection of normal saline.After successful establishment of the model,EA(2 Hz,2 mA)was applied to these acupoints for 15 min,once daily for 10 days.Then,the learning-memory ability was assessed by using Morris water maze tests,and the expression levels of TNF-αand IL-1βproteins in the PFC and hippocampus tissues were detected by using Western blot.Results Following modeling,the average escape latency of place navigation test were significantly increased(P<0.05)and the platform crossing times of spatial probe test was significantly decreased in the model group than in the control and sham operation groups(P<0.05).The expression levels of IL-1βand TNF-αproteins in the PFC and hippocampus were apparently up-regulated in the model group than in the control and the sham operation groups(P<0.0001,P<0.001,P<0.01).After the intervention,the increase of the average escape latency and expression of IL-1βand TNF-αin the PFC and hippocampus,and the decrease of space exploration test were revised in both GV20+BL23 EA and GV20+BL23+BL13 EA groups(P

关 键 词：电针 阿尔茨海默症 前额叶皮层 海马 学习记忆能力 白细胞介素1Β 肿瘤坏死因子Α 

分 类 号：R245.97[医药卫生—针灸推拿学]