基于网络药理学探讨中医药治疗脑梗死后“半身不遂”的作用机制  

Research on Mechanism of Traditional Chinese Medicine on"Hemiplegia"of Cerebral Infarction Based on Network Pharmacology

作　　者：谢丽华 何飘[1,2] 翟子豪 王小菊 谢姝杰 胡国恒 廖君[2] Xie Lihua;He Piao;Zhai Zihao;Wang Xiaoju;Xie Shujie;Hu Guoheng;Liao Jun(Hunan Unisersity of Chinese Medicire,Hunan,Changsha 410208,China)

机构地区：[1]湖南中医药大学,湖南长沙410228 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中国中医急症》2020年第8期1348-1351,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81573941,81774033);湖南中医药大学校级大学生研究性学习和创新性试验计划课题项目(1022|0001019132);湖南中医药大学中医学国内一流建设学科。

摘　　要：目的运用网络药理学方法,结合中医药临床或实验研究,探讨中医药治疗脑梗死后半身不遂的作用机制。方法借助人类基因数据库(GeneCards)、HPO数据库筛选脑梗死与半身不遂疾病靶点,运用STRING11.0数据库、Cytoscape3.6.1软件对靶点进行PPI网络构建,运用DAVID 6.8数据库对靶点进行基因本体(GO)富集、KEGG通路注释分析。结果对脑梗死与半身不遂现有的靶标进行分析后得到39个共有靶标,PPI网络图显示前10个联系最多的靶点是TP53、TNF、IL10、MMP9、SNCA、TGFB1、PTEN、MTOR、SOD1、ACTA2。GO富集和KEGG注释分析结果显示,中医药治疗脑梗死后半身不遂与抗氧化、抑制脑组织细胞凋亡、抑制炎症反应、促进血管再生、调节血脂代谢、抑制脑细胞自噬死亡、免疫调节等机制有关。结论中医药治疗脑梗死后半身不遂使通过上述潜在的"多靶点-多途径"来发挥作用,该研究为中医药治疗脑梗死后半身不遂作用机制的进一步研究奠定基础。Objective:To investigate the mechanism of traditional Chinese medicine on hemiplegia of cerebral infarction based on network pharmacology and clinical or experimental study of TCM.Methods:The targets of cerebral infarction and hemiplegia were screened by GeneCards database and HPO database.STRING 11.0 database and Cytoscape 3.6.1 software were used to construct PPI network of targets.DAVID 6.8 database was used for GO enrichment and KEGG pathway annotation analysis.Results:39 common targets were obtained after analyzing targets of cerebral infarction and hemiplegia.PPI network map showed that the top 10 targets were TP53,TNF,IL10,MMP9,SNCA,TGFB1,PTEN,MTOR,SOD1 and ACTA2.The results of GO enrichment and KEGG pathway enrichment analysis showed that TCM treatment of hemiplegia after cerebral infarction was related to antioxidation,inhibition of apoptosis of brain tissue cells,inhibition of inflammatory reaction,promotion of vascular regeneration,regulation of blood lipid metabolism,inhibition of autophagy death of brain cells,immune regulation and other mechanisms.Conclusion:TCM can play a role through the above potential"multi-target-multi-pathway"in the treatment of hemiplegia of cerebral infarction,which lays a foundation for further study of the mechanism of hemiplegia of cerebral infarction treated with TCM.

关 键 词：脑梗死 半身不遂 网络药理学 中医药 

分 类 号：R743.9[医药卫生—神经病学与精神病学]