GPIIIa PLA2、PEAR1、PTGS1、GP1BA基因多态性与阿司匹林抵抗的相关性研究  

Correlation between aspirin resistance and gene polymorphisms of GPIIIa PLA2,PEAR1,PTGS1,GP1BA

作　　者：陈莉[1] 严浩 毛士龙[1] Chen Li;Yan Hao;Mao Shilong(Department of Pharmacy,Central Hospital of Xuhui District,Shanghai 200031,China;Pharmacy College,Shanghai University of Medicine&Health Sciences,Shanghai 201318,China)

机构地区：[1]上海市徐汇区中心医院药剂科,上海200031 [2]上海健康医学院药学院,上海201318

出　　处：《医药前沿》2020年第16期14-16,共3页Journal of Frontiers of Medicine

基　　金：上海市临床药学重点专科建设项目(No.沪卫计药政[2018]9号);上海市徐汇区中心医院院级课题。

摘　　要：目的:探讨GPIIIa PLA2、PEAR1、PTGS1、GP1BA基因多态性与阿司匹林抵抗的相关性。方法:收集2016年12月-2019年7月就诊于上海市徐汇区中心医院,而且诊断为缺血性疾病并进行阿司匹林基因检测及血小板聚集率试验检测的患者,根据相关的标准进行筛选,最终选出1175例患者。依据花生四烯酸(arachidonic acid,AA)诱导的血小板聚集率数值将患者分为两组,分别是阿司匹林抵抗(aspirin resistance AR)组和阿司匹林敏感(aspirin sensitive,AS)组,分析阿司匹林抵抗的相关基因多态性。结果:AR者99例,发生率为8.43%,AS者1076例,发生率为91.57%;GPIIIa PLA2及PTGS1突变频率较低,突变率分别为0.85%和0.09%,GP1BA和PEAR1突变频率较高,突变率分别为9.28%和59.4%,且突变杂合型频率高于突变纯合型;通过基因检测结果的相关性分析发现PEAR1或GP1BA的基因多态性与AR有关,PEAR1突变型患者AR风险增加,GP1BA野生型患者阿司匹林抵抗的风险增加。AR组男性患者的比例、2型糖尿病发生率以及低密度脂蛋白水平显著性高于AS组(P<0.05)。结论:PEAR1和GP1BA基因多态性与AR相关,性别、合并2型糖尿病以及较高的低密度脂蛋白水平是导致AR的风险因素。Objectjve To investigate the relevance between GPIIIa PLA2,PEAR1,PTGS1,GP1BA gene polymorphisms and aspirin resistance(AR).Methods A total of 1297 patients who were diagnosed with ischemic disease,tested for aspirin related genes and detected the level of platelet aggregation rate induced by AA or ADP were selected retrospectively from December 2016 to July 2019 in Shanghai Xuhui District Central Hospital.GPIIIa PLA2 and PTGS1 gene polymorphisms were rare in Han Chinese population.Results There were 99 cases of AR(8.43%)and 1076 cases of as(91.57%).The frequency of GIIIa PLA2 and PTGS1 mutation were low,but the mutation frequency of GP1BA and PEAR1 was 9.28%and 59.4%,respectively.The mutation heterozygous frequencies were higher than mutant homozygotic types.The correlation analysis of gene sequencing results showed that the gene polymorphism of PEAR1 or GP1BA was associated with AR.The risk of AR increased in PEAR1 mutant patients and in GP1BA wild type patients.Compared with the clinical data of AS group,there were significant differences in the following three aspects(P<0.5):In AR group,the proportion of men was high,the incidence of type 2 diabetes mellitus was high,and the level of low density lipoprotein(LDL)was high.Conclusions PEAR1 and GP1BA gene polymorphism is associated with AR,in addition,sex,combined with type 2 diabetes as well as higher LDL levels are risk factors for AR.

关 键 词：阿司匹林抵抗 基因测序 基因多态性 血小板聚集 

分 类 号：R917[医药卫生—药物分析学]