基于SWCNTs的环丙沙星荧光增敏法研究  

Fluorescence Enhancement Method for Ciprofloxacin Extraction by Modified Products of SWCNTs

作　　者：胡雅稳 秦铭杉 刘星妍 杨娇 杨琳燕 Hu Yawen;Qin Mingshan;Liu Xingyan;Yang Jiao;Yang Linyan(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院动物科学与动物医学学院,天津市农业动物繁育与健康养殖重点实验室,天津300384

出　　处：《南开大学学报（自然科学版）》2020年第4期99-107,共9页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：Supported by the Outstanding Research Achievement Award Project of Tianjin Agricultural University;the National Natural Science Foundation of China(21875117,31572492);the Innovative and Entrepreneurial Training Plan for Tianjin College Students(202010061066);the Veterinary“Innovative Talent People of Young and Middle Age Key Members Project”of Tianjin;the Natural Science Foundation of Tianjin(18JCYBJC30100)。

摘　　要：SWCNTs-PEG-NH2,SWCNTs-2,3-NH2和SWCNTs-3,4-NH2纳米微球可经由单壁碳纳米管(SWCNTs)共价修饰制备.SWCNTs-PEG-NH2,SWCNTs-2,3-NH2和SWCNTs-3,4-NH2纳米微球的结构可由X-射线光电子能谱(XPS)确证.结果表明酰胺化反应成功.环丙沙星(CIP)萃取实验表明,对于SWCNTs-PEG-NH2微球,在pH=4和pH=6得到较好吸附效果,而0.1 mol/L NaOH和1%SDS洗脱剂可得到较好的洗脱效果.对于SWCNTs-2,3-NH2和SWCNTs-3,4-NH2微球,pH=6得到最好的吸附效果,而1%SDS洗脱条件可到最好的洗脱效果.Al3+离子的加入可以敏化环丙沙星的荧光.SWCNTs-PEG-NH2,SWCNTs-2,3-NH2 and SWCNTs-3,4-NH2 nanoparticles could be prepared by covalent modification method of single-walled carbon nanotubes(SWCNTs).The structure of SWCNTs-PEG-NH2,SWCNTs-2,3-NH2 and SWCNTs-3,4-NH2 nanoparticles could be determined by XPS(X-ray photoelectron spectroscopy).The results showed that the amidation reactions were successful.Ciprofloxacin(CIP)extraction experiments exhibited that,as for SWCNTs-PEG-NH2,the best adsorption could be obtained at pH=4 and pH=6,while elution conditions of 0.1mol/L NaOH and 1%SDS could achieve better elution effect.As for SWCNTs-2,3-NH2 and SWCNTs-3,4-NH2,the best adsorption could be obtained at pH=6,while the elution condition of 1%SDS could achieve the best elution effect.The addition of Al3+ions could sensitize the fluorescence of ciprofloxacin.

关 键 词：SWCNTS 环丙沙星 修饰 萃取 

分 类 号：R914.2[医药卫生—药物化学]